Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AZSP09_RS11510 Genome accession   NZ_AP021844
Coordinates   2455339..2456817 (-) Length   492 a.a.
NCBI ID   WP_152090117.1    Uniprot ID   A0A5H2YD09
Organism   Azospira sp. I09     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2450339..2461817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZSP09_RS11475 (AZSP09_22660) - 2450599..2451408 (-) 810 WP_197739011.1 hypothetical protein -
  AZSP09_RS11480 (AZSP09_22670) - 2451471..2452286 (-) 816 WP_152090115.1 hypothetical protein -
  AZSP09_RS11485 (AZSP09_22680) - 2452303..2452974 (-) 672 WP_130459699.1 hypothetical protein -
  AZSP09_RS11490 (AZSP09_22690) - 2453099..2453413 (-) 315 WP_152090116.1 PsiF family protein -
  AZSP09_RS11495 (AZSP09_22700) hprK 2453459..2454394 (-) 936 WP_014235811.1 HPr(Ser) kinase/phosphatase -
  AZSP09_RS11500 (AZSP09_22710) ptsN 2454363..2454848 (-) 486 WP_014235812.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AZSP09_RS11505 (AZSP09_22720) raiA 2455004..2455324 (-) 321 WP_014235813.1 ribosome-associated translation inhibitor RaiA -
  AZSP09_RS11510 (AZSP09_22730) rpoN/rpoN1 2455339..2456817 (-) 1479 WP_152090117.1 RNA polymerase factor sigma-54 Machinery gene
  AZSP09_RS11515 (AZSP09_22740) lptB 2456884..2457630 (-) 747 WP_014235815.1 LPS export ABC transporter ATP-binding protein -
  AZSP09_RS11520 (AZSP09_22750) recX 2457696..2458151 (-) 456 WP_130459696.1 recombination regulator RecX -
  AZSP09_RS11525 (AZSP09_22760) recA 2458148..2459170 (-) 1023 WP_014235817.1 recombinase RecA Machinery gene
  AZSP09_RS11530 (AZSP09_22770) - 2459307..2461388 (+) 2082 WP_172974749.1 PAS domain S-box protein -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 55220.03 Da        Isoelectric Point: 4.7048

>NTDB_id=74937 AZSP09_RS11510 WP_152090117.1 2455339..2456817(-) (rpoN/rpoN1) [Azospira sp. I09]
MKPTLQLKLSQHLALTPQLQQSIRLLQLSTLELEQELEKYLLENPLLEREDENYEPSYSGSEGEYAGSTAESAAEGPREG
DSGESGEGADSHGPEEVRDDSRDRLGEGEDWFAEGSYPSASRDEDEDSDFQEVQAANTSLRDHLNWQLGLMQLSERDRQL
VSFLIEALDEDGYLTQPLEELQEALPEELEIDLDDLQIALRHLQHLDPTGVGARTPGECLALQLEALPADEVRDQALKLV
RHHLELLAGRDFTKLKKLLGCDDERLRAVTNLVKSLNPRPGAQYAPLDARYITPDVVVKKVRNQWTASINPDAYPRLRIN
RLYAEILSKQRGSSLSGQLQEARWLIKNVQQRFDTILRVSQAILDRQRQFFEHGEVAMRPLVLREIAETLGLHESTVSRV
TTQKYMATPRGIFELKYFFGSHVATDTGGACSATAIRALIKQLVAAEDPKKPLSDSQISEILGQQGIVVARRTIAKYREA
LNISPANLRKSI

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=74937 AZSP09_RS11510 WP_152090117.1 2455339..2456817(-) (rpoN/rpoN1) [Azospira sp. I09]
ATGAAACCAACCCTCCAACTCAAACTCTCGCAGCACTTGGCCCTGACGCCACAGCTGCAGCAGTCCATCCGGCTGCTGCA
ACTGTCGACGCTTGAGCTGGAGCAGGAGTTGGAGAAGTATCTGCTGGAAAACCCCCTGCTGGAGCGGGAGGACGAGAACT
ACGAGCCCTCCTACAGCGGGAGCGAAGGCGAATACGCCGGCAGTACGGCCGAGTCCGCTGCCGAAGGCCCGCGGGAAGGC
GATAGCGGCGAATCCGGTGAAGGCGCCGACAGTCACGGCCCCGAAGAAGTCCGGGACGACAGCCGGGACCGCCTGGGTGA
GGGCGAGGACTGGTTTGCCGAAGGCAGCTACCCTTCCGCCTCCCGCGACGAGGACGAGGATTCCGACTTCCAGGAAGTGC
AGGCCGCCAACACCTCCCTGCGCGACCACCTCAACTGGCAGCTGGGCCTGATGCAGCTCTCCGAGCGGGACCGGCAGCTG
GTCAGCTTCCTGATCGAGGCGCTGGACGAGGACGGCTACCTGACCCAGCCCCTGGAGGAGCTGCAGGAAGCCCTGCCGGA
AGAGCTGGAAATCGATCTGGACGACCTGCAGATCGCCCTGCGCCACCTGCAGCACCTGGACCCGACCGGTGTCGGTGCCC
GCACTCCGGGCGAATGCCTGGCCCTGCAGCTGGAAGCCCTGCCGGCCGACGAGGTGCGGGACCAGGCCCTGAAGCTGGTG
CGCCATCACCTGGAACTGCTGGCCGGCCGGGACTTCACCAAGCTGAAGAAGCTGCTGGGCTGCGACGACGAGCGCCTGCG
CGCCGTCACCAACCTGGTGAAGAGCCTCAACCCCCGGCCCGGCGCCCAGTACGCGCCCCTGGATGCCCGCTACATCACCC
CGGACGTGGTGGTGAAGAAGGTGCGCAACCAGTGGACCGCCAGCATCAATCCGGACGCCTACCCGCGCCTGCGCATCAAC
CGGCTCTACGCCGAGATCCTCTCCAAGCAGCGCGGCTCCAGCCTCTCCGGCCAGCTGCAGGAGGCCCGCTGGCTGATCAA
GAACGTGCAGCAGCGTTTCGACACCATCCTGCGCGTGTCCCAGGCCATCCTGGACCGCCAGCGCCAGTTCTTCGAACACG
GCGAGGTGGCCATGCGTCCCCTGGTACTGCGGGAAATCGCCGAAACCCTGGGCCTGCACGAATCCACCGTCTCCCGGGTG
ACCACCCAGAAGTACATGGCCACCCCCCGGGGCATTTTCGAACTCAAGTATTTCTTCGGCAGCCACGTGGCCACGGATAC
GGGGGGCGCCTGTTCGGCCACCGCCATCCGGGCCCTGATCAAGCAGCTGGTGGCGGCCGAAGACCCCAAAAAACCCCTTT
CGGACAGTCAAATATCCGAAATTCTTGGCCAACAAGGCATTGTTGTGGCGCGTCGAACCATTGCAAAATACAGAGAAGCG
CTCAACATTTCGCCGGCCAACCTACGCAAGTCCATCTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-490)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H2YD09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.199

100

0.589

Multiple sequence alignment