Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   OHK33_RS12530 Genome accession   NZ_CP109770
Coordinates   2803814..2805196 (+) Length   460 a.a.
NCBI ID   WP_012773160.1    Uniprot ID   A0AAW3SUS5
Organism   Pectobacterium aroidearum strain T25-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2798814..2810196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHK33_RS12510 (OHK33_12505) prfC 2799102..2800691 (-) 1590 WP_180778879.1 peptide chain release factor 3 -
  OHK33_RS12515 (OHK33_12510) - 2800992..2801909 (+) 918 WP_350315704.1 hypothetical protein -
  OHK33_RS12520 (OHK33_12515) - 2801964..2802662 (-) 699 WP_350315705.1 YtjB family periplasmic protein -
  OHK33_RS12525 (OHK33_12520) serB 2802819..2803796 (+) 978 WP_182100404.1 phosphoserine phosphatase -
  OHK33_RS12530 (OHK33_12525) radA 2803814..2805196 (+) 1383 WP_012773160.1 DNA repair protein RadA Machinery gene
  OHK33_RS12535 (OHK33_12530) nadR 2805328..2806581 (+) 1254 WP_012773159.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  OHK33_RS12540 (OHK33_12535) - 2806578..2807219 (-) 642 WP_350315706.1 5-formyltetrahydrofolate cyclo-ligase -
  OHK33_RS12550 (OHK33_12545) zapA 2807517..2807846 (-) 330 WP_012773157.1 cell division protein ZapA -
  OHK33_RS12555 (OHK33_12550) - 2808039..2808626 (+) 588 WP_012773156.1 YecA family protein -
  OHK33_RS12560 (OHK33_12555) pepP 2808717..2810036 (+) 1320 WP_350317178.1 Xaa-Pro aminopeptidase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49608.13 Da        Isoelectric Point: 7.4218

>NTDB_id=749134 OHK33_RS12530 WP_012773160.1 2803814..2805196(+) (radA) [Pectobacterium aroidearum strain T25-1]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASVSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDQ
SMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKTPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=749134 OHK33_RS12530 WP_012773160.1 2803814..2805196(+) (radA) [Pectobacterium aroidearum strain T25-1]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGATTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAACACCATTACCGAAGTGCGTCTGGCATCGGCGTCAGTATCACGTTCCGACCGCCTCACCGGCTATG
CGGGTGAGAGTGCTGGCGTCAGCCGGGTACAGAAACTTTCGGAAATCAGCCTTGAAGCCCTGCCCCGTTTTTCTACCGGT
TTTCAGGAGTTTGACCGCGTTTTGGGCGGCGGCGTCGTTCCCGGCAGCGCGATTCTGATCGGCGGTAACCCCGGCGCAGG
TAAAAGTACCCTGCTGCTGCAAACGCTCTGCAAGCTGTCAGAGAATATGAAAACCCTGTACGTCACCGGGGAAGAATCCT
TACAGCAGGTGGCGATGCGGGCACACCGCCTCAATTTACCGACCCAGAATCTCAATATGCTGTCGGAAACCAGCATCGAA
CAAATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGTTGATGGTGATCGACTCCATTCAGGTCATGCATCTCGCCGATAT
TCAATCGTCCCCCGGCAGTGTAGCGCAGGTGCGAGAAACCGCAGCCTACCTGACGCGCTTTGCCAAAACGCGCGGCGTCG
CCATCGTCATGGTCGGCCACGTCACCAAGGACGGCTCGCTCGCCGGACCGAAAGTATTAGAACACTGCATCGACTGCTCC
GTGTTGCTGGATGGCGATGCCGATTCCCGCTTCCGCACCCTGCGCAGCCATAAAAACCGTTTCGGTGCCGTTAACGAACT
GGGCGTGTTCGCGATGACGGAGCAAGGACTACGCGAGGTCAGCAATCCGTCGGCGATTTTCCTTAGCCGCGGGGACGAAG
TGACGTCCGGCAGTTCCGTTATGGTGGTGTGGGAAGGCACGCGCCCGCTGCTGGTCGAGATTCAGGCGCTGGTGGATCAA
TCGATGATGTCGAACCCACGCCGTGTGGCGGTCGGGCTGGAGCAAAATCGGTTAGCCATACTGCTAGCGGTGCTGCATCG
CCACGGCGGCTTGCAGATGTCAGATCAGGATGTGTTCGTAAATGTCGTCGGCGGCGTCAAAGTCACCGAAACCAGCGCCG
ATCTGGCGCTGCTGTTGTCACTGGTCTCCAGCTTCCGCGACCGCCCGCTGCCACAGGATCTCGTCATCTTCGGTGAAGTC
GGTCTGGCGGGCGAAATCCGCCCGGTTCCCAGCGGACAAGAGCGGATTACCGAAGCCGCCAAGCACGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAACGCCTGCCAGTATGCAGGTATTCGGCGTGAAAAAGCTGGCCGACGCGC
TGGCGATCTTAGACGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.696

100

0.487
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis SK321

43.421

99.13

0.43