Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   OHU75_RS09645 Genome accession   NZ_CP109761
Coordinates   1764786..1765838 (-) Length   350 a.a.
NCBI ID   WP_013059177.1    Uniprot ID   A0A8D4BHW7
Organism   Priestia megaterium strain P-NA14     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1759786..1770838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHU75_RS09605 (OHU75_09605) - 1760802..1761626 (+) 825 WP_028410647.1 YqhG family protein -
  OHU75_RS09610 (OHU75_09610) - 1761650..1761835 (-) 186 WP_013059170.1 YqzE family protein -
  OHU75_RS09615 (OHU75_09615) comGG 1761879..1762253 (-) 375 WP_098579241.1 competence type IV pilus minor pilin ComGG -
  OHU75_RS09620 (OHU75_09620) comGF 1762243..1762680 (-) 438 WP_013059172.1 competence type IV pilus minor pilin ComGF -
  OHU75_RS09625 (OHU75_09625) - 1762677..1763015 (-) 339 WP_028410645.1 type II secretion system protein -
  OHU75_RS09630 (OHU75_09630) comGD 1762999..1763481 (-) 483 WP_255266894.1 competence type IV pilus minor pilin ComGD -
  OHU75_RS09635 (OHU75_09635) comGC 1763423..1763734 (-) 312 WP_098579240.1 competence type IV pilus major pilin ComGC -
  OHU75_RS09640 (OHU75_09640) comGB 1763749..1764795 (-) 1047 WP_028410643.1 competence type IV pilus assembly protein ComGB -
  OHU75_RS09645 (OHU75_09645) comGA 1764786..1765838 (-) 1053 WP_013059177.1 competence type IV pilus ATPase ComGA Machinery gene
  OHU75_RS09650 (OHU75_09650) - 1766106..1766489 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  OHU75_RS09655 (OHU75_09655) - 1766751..1766987 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  OHU75_RS09660 (OHU75_09660) - 1767025..1767657 (-) 633 WP_028410642.1 MBL fold metallo-hydrolase -
  OHU75_RS09665 (OHU75_09665) - 1767866..1768042 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  OHU75_RS09670 (OHU75_09670) - 1768078..1768980 (-) 903 WP_028410641.1 M14 family metallocarboxypeptidase -
  OHU75_RS09675 (OHU75_09675) - 1769092..1770060 (-) 969 WP_098579239.1 ROK family glucokinase -
  OHU75_RS09680 (OHU75_09680) - 1770063..1770269 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39625.02 Da        Isoelectric Point: 9.0586

>NTDB_id=749040 OHU75_RS09645 WP_013059177.1 1764786..1765838(-) (comGA) [Priestia megaterium strain P-NA14]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=749040 OHU75_RS09645 WP_013059177.1 1764786..1765838(-) (comGA) [Priestia megaterium strain P-NA14]
TTGACCGAAATTGAACAGTTTGCAACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAACGAAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTACGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCATCTTTTTAATCGAAATGTTATTACACTAGAAGACCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGCGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCATCCCGCTTGTAAAAAAATGAGAA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586