Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   N8A73_RS11275 Genome accession   NZ_CP109651
Coordinates   2204946..2206016 (-) Length   356 a.a.
NCBI ID   WP_041117979.1    Uniprot ID   -
Organism   Bacillus safensis strain LG01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2199946..2211016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A73_RS11225 sipW 2200040..2200612 (-) 573 WP_034280218.1 signal peptidase I SipW -
  N8A73_RS11230 tapA 2200665..2201192 (-) 528 WP_041117967.1 amyloid fiber anchoring/assembly protein TapA -
  N8A73_RS11235 - 2201492..2201770 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  N8A73_RS11240 - 2201805..2201999 (-) 195 WP_024422974.1 YqzE family protein -
  N8A73_RS11245 - 2202059..2202442 (-) 384 WP_041117969.1 competence type IV pilus minor pilin ComGG -
  N8A73_RS11250 comGF 2202439..2202966 (-) 528 WP_264315971.1 competence type IV pilus minor pilin ComGF -
  N8A73_RS11255 comGE 2202878..2203192 (-) 315 WP_264315972.1 competence type IV pilus minor pilin ComGE -
  N8A73_RS11260 comGD 2203176..2203622 (-) 447 WP_176966991.1 competence type IV pilus minor pilin ComGD -
  N8A73_RS11265 comGC 2203615..2203908 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  N8A73_RS11270 comGB 2203925..2204965 (-) 1041 WP_034280202.1 competence type IV pilus assembly protein ComGB -
  N8A73_RS11275 comGA 2204946..2206016 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  N8A73_RS11280 - 2206194..2207069 (-) 876 WP_024422979.1 STAS domain-containing protein -
  N8A73_RS11290 - 2207293..2207673 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  N8A73_RS11295 - 2207886..2208128 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  N8A73_RS11300 - 2208173..2208811 (-) 639 WP_176966990.1 MBL fold metallo-hydrolase -
  N8A73_RS11305 - 2208969..2209142 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  N8A73_RS11310 - 2209166..2209480 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  N8A73_RS11315 - 2209557..2210810 (-) 1254 WP_176966989.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40398.79 Da        Isoelectric Point: 9.4061

>NTDB_id=748748 N8A73_RS11275 WP_041117979.1 2204946..2206016(-) (comGA) [Bacillus safensis strain LG01]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=748748 N8A73_RS11275 WP_041117979.1 2204946..2206016(-) (comGA) [Bacillus safensis strain LG01]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAGAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643