Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   N8A73_RS01905 Genome accession   NZ_CP109651
Coordinates   354299..354727 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus safensis strain LG01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 349299..359727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A73_RS01875 - 349463..349792 (+) 330 WP_024424311.1 YckD family protein -
  N8A73_RS01880 - 349836..350600 (-) 765 WP_176967338.1 MBL fold metallo-hydrolase -
  N8A73_RS01885 - 350716..351132 (+) 417 WP_176967337.1 Lrp/AsnC family transcriptional regulator -
  N8A73_RS01890 - 351161..351967 (+) 807 WP_254001557.1 aminoglycoside N(3)-acetyltransferase -
  N8A73_RS01895 - 351969..352355 (-) 387 WP_041106618.1 competence protein ComJ -
  N8A73_RS01900 - 352475..354172 (-) 1698 WP_151863131.1 methyl-accepting chemotaxis protein -
  N8A73_RS01905 nucA/comI 354299..354727 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  N8A73_RS01910 - 355095..357164 (+) 2070 WP_254001558.1 hydantoinase/oxoprolinase family protein -
  N8A73_RS01915 - 357154..359142 (+) 1989 WP_264316033.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=748716 N8A73_RS01905 WP_024424305.1 354299..354727(-) (nucA/comI) [Bacillus safensis strain LG01]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=748716 N8A73_RS01905 WP_024424305.1 354299..354727(-) (nucA/comI) [Bacillus safensis strain LG01]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTAGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCATCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCAATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATTCAATTTATCATTGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535