Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   OF801_RS01145 Genome accession   NZ_CP109635
Coordinates   213836..215197 (+) Length   453 a.a.
NCBI ID   WP_264308686.1    Uniprot ID   A0AA46YVK7
Organism   Lactococcus garvieae strain Alexii-11_2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 208120..213615 213836..215197 flank 221


Gene organization within MGE regions


Location: 208120..215197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OF801_RS01115 (OF801_01115) - 209857..210801 (+) 945 WP_264308685.1 ZmpA/ZmpB/ZmpC family metallo-endopeptidase-related protein -
  OF801_RS01120 (OF801_01120) - 210776..211485 (-) 710 Protein_213 transposase -
  OF801_RS01125 (OF801_01125) - 211588..211875 (+) 288 WP_264308333.1 hypothetical protein -
  OF801_RS01130 (OF801_01130) - 211937..212149 (+) 213 WP_264308334.1 DNA-binding protein -
  OF801_RS01135 (OF801_01135) - 212218..213384 (+) 1167 WP_264308335.1 site-specific integrase -
  OF801_RS01145 (OF801_01145) radA 213836..215197 (+) 1362 WP_264308686.1 DNA repair protein RadA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49782.01 Da        Isoelectric Point: 4.9969

>NTDB_id=748631 OF801_RS01145 WP_264308686.1 213836..215197(+) (radA) [Lactococcus garvieae strain Alexii-11_2]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGNGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLKVVGVTTLSECLKKIFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=748631 OF801_RS01145 WP_264308686.1 213836..215197(+) (radA) [Lactococcus garvieae strain Alexii-11_2]
ATAGCTAAAAAAAAATCAACATTTCTTTGCCAGGAATGTGGCTACAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGTGCTTGGGGTTCCTTTGTTGAAGAAGTAGAAGTCCAAGAGGTAAAAAACCAGAGAGTGAGTCTGACAGGCGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTCGATACACCGCGTGTGGAAACCGATCTGGATGAGTTTAACCGCGTC
CTTGGAGGTGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGGGATCCAGGGATTGGTAAATCTACTCTACTGCTTCA
AGTATCTACGCAACTTGCTTCAAGAGGGCGTGTACTTTATGTCAGTGGGGAGGAGTCGGCTCAACAAATTAAGCTGCGTG
CCGAGCGTTTGGGCGACATCGATACAGATTTTTACCTTTACGCCGAAACCAATATGCAAAGCATCCGAAATGAAGTTGAA
CGTCTACAGCCAGACTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATCCAGAGTACTCAGGGTTCGGT
TAGTCAGGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACCAACGATATTGCGACCTTTATTGTCGGACATG
TCACTAAGGAAGGGCAACTTGCGGGGCCACGTATGTTGGAACATATGGTTGATACGGTCTTGTATTTTGAGGGAGAACGT
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGGATTTTCGAGATGCAGGG
CAATGGTTTAGTGGAAGTGACCAACCCGAGTGAAGTTTTTCTTGAGGAGCGTTTGGAGGGCTCTACGGGCTCTGCTATCG
TCTGTGCGTTAGAAGGTACGCGTCCGATATTGGTTGAGATTCAAGCTTTAACAACGCCAACCATGTTTGGAAATGCCAAA
CGTACAACCTCTGGTTTGGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTCGAAAAGCGTGCCGGTTTTCTTTTGCA
ACAACAAGATGCCTACTTAAAATCCGCAGGTGGTGTCAAGTTGGATGAACCAGCCATTGACTTAGCAGTTGCTGTAGCTA
TTGCTTCTAGCTACAAGGAAGAGCCGACAGATGCACGCGAATGCTTTATTGGCGAAATTGGCCTGACGGGTGAAATTCGT
CGTGTTACGCGGATGGAGCAACGTCTCAATGAAGCAAGCAAATTAGGGTTCCAAAAAGTTTACGCGCCTAAGAATAGCTT
GGCAGGCGTAGACATTCCAGAAAATTTGAAAGTGGTCGGTGTGACGACTTTATCAGAATGTTTGAAAAAAATATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.132

100

0.801
  radA Streptococcus pneumoniae D39

80.132

100

0.801
  radA Streptococcus pneumoniae R6

80.132

100

0.801
  radA Streptococcus pneumoniae TIGR4

80.132

100

0.801
  radA Streptococcus mitis NCTC 12261

80.132

100

0.801
  radA Streptococcus mitis SK321

80.132

100

0.801
  radA Bacillus subtilis subsp. subtilis str. 168

61.22

100

0.62