Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OE059_RS10310 Genome accession   NZ_CP109617
Coordinates   1965175..1966212 (-) Length   345 a.a.
NCBI ID   WP_015881136.1    Uniprot ID   -
Organism   Exiguobacterium profundum strain TSS-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1960175..1971212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OE059_RS10285 (OE059_10305) - 1960194..1960901 (-) 708 WP_275059957.1 hypothetical protein -
  OE059_RS10290 (OE059_10310) pilM 1960898..1962472 (-) 1575 WP_275059958.1 pilus assembly protein PilM -
  OE059_RS10295 (OE059_10315) - 1962485..1963237 (-) 753 WP_275059959.1 prepilin peptidase -
  OE059_RS10300 (OE059_10320) - 1963321..1963809 (-) 489 WP_214730815.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OE059_RS10305 (OE059_10325) - 1963951..1965168 (-) 1218 WP_214730813.1 type II secretion system F family protein -
  OE059_RS10310 (OE059_10330) pilT 1965175..1966212 (-) 1038 WP_015881136.1 type IV pilus twitching motility protein PilT Machinery gene
  OE059_RS10315 (OE059_10335) - 1966205..1967875 (-) 1671 WP_143404754.1 GspE/PulE family protein -
  OE059_RS10320 (OE059_10340) - 1967877..1968167 (-) 291 WP_078146549.1 hypothetical protein -
  OE059_RS10325 (OE059_10345) - 1968164..1969384 (-) 1221 WP_214872830.1 G5 domain-containing protein -
  OE059_RS10330 (OE059_10350) - 1969542..1969997 (+) 456 WP_047795513.1 type IV pilus modification PilV family protein -
  OE059_RS10335 (OE059_10355) - 1969945..1970508 (+) 564 WP_230872789.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38431.24 Da        Isoelectric Point: 6.4348

>NTDB_id=748613 OE059_RS10310 WP_015881136.1 1965175..1966212(-) (pilT) [Exiguobacterium profundum strain TSS-3]
MINQSIETILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTDDMRERLEHDRDLDFSFGVPR
VSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKRLIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIIT
LEDPIEYLHSHDQSIVDQREIGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRNEKEFQLPTVIQTSRQHGMQS
MDAAIEQLVRQGIINPNAVPTEEMR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=748613 OE059_RS10310 WP_015881136.1 1965175..1966212(-) (pilT) [Exiguobacterium profundum strain TSS-3]
ATGATTAATCAATCGATTGAAACCATTTTGACGAATGCGGTCGAGAAACGTGCCTCGGACATTCACCTGACAGCCGGTTC
CCCACCGATTATTCGAGTAGATGGGAGTCTCATTACGATGGGCGAGGAGAAGGTATTACCCCACCATCTTGAAGGATATT
TAAAAGAAATTATGACCGATGATATGCGCGAACGTCTCGAGCATGATCGGGATCTCGATTTCTCGTTCGGTGTCCCACGT
GTTTCCCGTTTTCGGGTCAACGCCTACTATCAACGAGGCACGATTGCCATCGCATTCCGATCCATTTCCGGGGAAATCCC
GACGTTGAATGAACTCAATCTGCCGACTGTGTTGAAGCGTTTGATTGAAAAACCACATGGTCTCATCTTGGTCACGGGTC
CGACAGGGTCAGGGAAATCAACGACGCTCGCTGCGATGATTCATGAGATTAACCGCACGCAGCGAAAGCGGATCATCACG
CTAGAAGATCCGATCGAGTATTTGCATTCCCACGACCAGAGTATTGTCGACCAACGGGAAATCGGCTCTGACGTCATGAA
ATTTGTGAGTGGTCTACGGGCTGCCTTACGGCAAGACCCAGACGTCATTCTGCTTGGGGAGATGCGGGACTTAGAAACGA
TTTCGACGGCGATGACCGCCGCAGAGACGGGGCACCTCGTCATGGCAACGCTCCATACGTCGAGTGCGATGTCGACGGTG
AGTCGAATCATCGACGCGTTCCCAGCTGAGCAACAGGACCAAATCCGGACACAACTCGCCAACGTCCTGCTTGGCGTCGT
GAGTCAACGGTTATTTCCACGGATTGATGTGCCGAAAGGACGCGTCGCCGCCATGGAAATCATGTTGGGGAATCCGGCTG
TTGCCAACTTGATTCGAAATGAAAAAGAGTTCCAGTTGCCGACCGTCATCCAGACGAGTCGCCAGCACGGGATGCAGTCG
ATGGATGCCGCCATCGAACAACTCGTTCGGCAAGGGATCATCAATCCAAACGCCGTTCCGACAGAAGAGATGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.76

95.362

0.484
  pilT Acinetobacter baylyi ADP1

48.788

95.652

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.181

97.681

0.461
  pilT Vibrio cholerae strain A1552

47.181

97.681

0.461
  pilT Pseudomonas aeruginosa PAK

47.879

95.652

0.458
  pilT Acinetobacter baumannii D1279779

47.024

97.391

0.458
  pilT Acinetobacter nosocomialis M2

47.024

97.391

0.458
  pilT Acinetobacter baumannii strain A118

47.024

97.391

0.458
  pilT Neisseria meningitidis 8013

47.289

96.232

0.455
  pilT Neisseria gonorrhoeae MS11

46.547

96.522

0.449
  pilT Legionella pneumophila strain Lp02

46.667

95.652

0.446
  pilT Legionella pneumophila strain ERS1305867

46.667

95.652

0.446
  pilT Pseudomonas stutzeri DSM 10701

45.946

96.522

0.443
  pilU Vibrio cholerae strain A1552

41.463

95.072

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.426

95.362

0.386