Detailed information NTG0744628

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QBD03_RS05345
• Genome accession: NZ_CP122959
• Coordinates: 1097787..1101521 (+)
• Length: 1244 a.a.
• NCBI ID: WP_280102477.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain HUP1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1092787..1106521

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QBD03_RS05335 (QBD03_05335)	mvk	1093006..1093971 (-)	966	WP_280102475.1	mevalonate kinase	-
QBD03_RS05340 (QBD03_05340)	-	1094234..1097794 (+)	3561	WP_280102476.1	PD-(D/E)XK nuclease family protein	-
QBD03_RS05345 (QBD03_05345)	addA	1097787..1101521 (+)	3735	WP_280102477.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QBD03_RS05350 (QBD03_05350)	-	1101587..1104421 (+)	2835	WP_280102478.1	helicase C-terminal domain-containing protein	-
QBD03_RS05355 (QBD03_05355)	-	1104499..1104975 (+)	477	WP_011374615.1	DUF5590 domain-containing protein	-
QBD03_RS05360 (QBD03_05360)	asnS	1105086..1106384 (+)	1299	WP_280102479.1	asparagine--tRNA ligase	-

Sequence

Protein

• Length: 1244 a.a.
• Molecular weight: 141407.22 Da
• Isoelectric Point: 4.7544

>NTDB_id=744628 QBD03_RS05345 WP_280102477.1 1097787..1101521(+) (addA) [Latilactobacillus sakei strain HUP1]
MSNPQFTPSQQAAVDHDGHDILVSASAGSGKTSVLVARVIQKILKGTDVDTLLVVTFTEAAATEMRQRIQAALRDASEKA
TEPAVKQRLRQQLSLVPTAQISTLHAFCLKVIKQFYYVIDRDPVFRLLSDTAERLLLADQVWQRVREAFYNHEYVKENEQ
DTLFYELAQNFSNDRNDDGLTDIVFELLDFANANPNPVKWLNKLPKSYAVDEAGLTASDYFQEKILPILQQIITECLEAL
QEAEQLSQTSENLMIYTPQIATTQAALTQINADAKTLNWQAWRQQLTDAQLGPAKRTKKLEPDEQINKDRLKADLDDVKK
KIQTLLDTYFVFDEATTANIQRQAGQLVQKLVSVTLTFRAAFQAEKERRHLLDFSDLEQLCLTILSVEDSPARAFYQQKF
SEVLVDEYQDTNPLQETIIQKVTSDHPRNLFMVGDVKQSIYAFRLADPSLFKNKYNEFGVTEDERDSERIILAENFRSRR
NIDDFTNLIFKQLMDENLGELDYDENAALQYGARYYPDQHPTAPTELLLYETKPEEAVANPQLDKSEGQVVAVAKRIQAM
MTNGEQIWDKKLEKMRPIEYRDIVLLAPTRGNNLFILDYFKRFGLPVVIKDAQNYFQTTEVQIMLALLQVIDNPNQDISL
VSVLRSPIVGLNENELALIRINDKTDDYYQAVFNFIDQYNEQKVGHLGQQVMQKLTHFMALLTSFRTIARQQPIVDLIWT
IYQETGFLDYVGGMPAGRQRQANLHALYERATAYEENGFKGLFQFIQFIERLQKQDKDLAQPTSLENQDAISVMTIHGSK
GLEFPVVFLIDTSRRFNQQDLQRSYVLDNHGGLGVVYLDSQKRLKVPTLPELAITAQKRKKLRAEEMRKLYVALTRAEQR
LIIVGHCDNQAQLMKQWQKGQNSTQQILDDGVRNDAASLLDWIGLSLIRHPQAKDWTTDYEPLLALSGDQTEFKLIMTNE
TLLGEIPGSLHNDQDWGQQQQKALQTIDLNSGIATAIAEQLTFKYPANVATQTTAYQSVSEVKRLFEDPDNTGLGQLDLA
AEQPRQANRYVTDSLVQPQFLQTVQQPTAAEVGTATHLVLQEIDLTQPVTLASLQALVDRLVLQNSLSANVAAKINLNHL
LTFFESDLGQQLLAHPEAVKREVPFSLLLPAETIFEGIRPTDDDHVLIHGIMDGYLLTDDGCYLFDYKTDFIDPSDQEAA
IERVKTRYAGQINLYSAALNQIAQKPVTHKYLYLLSIGELVEIN

Nucleotide

• Length: 3735 bp

>NTDB_id=744628 QBD03_RS05345 WP_280102477.1 1097787..1101521(+) (addA) [Latilactobacillus sakei strain HUP1]
TTGAGTAACCCCCAATTTACACCTTCACAGCAAGCCGCTGTCGATCATGATGGCCACGATATTTTAGTATCCGCATCAGC
TGGTTCAGGGAAGACATCCGTTCTTGTTGCACGGGTCATCCAGAAAATCTTAAAAGGGACTGATGTCGACACCTTACTTG
TTGTGACCTTTACGGAAGCCGCTGCTACTGAAATGCGCCAACGGATTCAAGCAGCACTACGCGATGCTAGCGAGAAAGCG
ACAGAGCCAGCCGTTAAACAGCGCTTACGGCAACAATTAAGTCTCGTGCCTACCGCACAAATCAGTACACTGCATGCCTT
TTGTTTAAAAGTCATTAAACAATTTTATTATGTCATTGATCGTGATCCCGTATTCCGCTTATTGAGTGATACCGCAGAAC
GACTCCTATTAGCGGATCAAGTATGGCAACGGGTGAGAGAGGCCTTCTATAATCACGAATATGTCAAAGAAAATGAGCAA
GATACATTATTCTACGAATTAGCCCAAAATTTCTCGAATGATCGTAACGATGACGGCCTAACCGACATTGTTTTTGAGTT
GCTCGATTTTGCAAACGCCAATCCCAACCCGGTTAAATGGTTAAATAAACTACCTAAGAGTTATGCTGTTGATGAAGCTG
GTTTGACTGCCAGTGATTATTTCCAAGAAAAAATATTACCCATTTTACAGCAAATCATCACCGAATGCTTGGAAGCCTTA
CAAGAAGCTGAACAGCTCAGTCAAACGAGCGAAAATCTAATGATTTATACCCCTCAAATTGCAACTACGCAAGCAGCCTT
AACACAAATTAACGCAGATGCAAAAACGTTGAATTGGCAAGCATGGCGCCAACAACTAACAGATGCACAGCTGGGGCCTG
CTAAACGGACTAAGAAATTAGAACCCGACGAACAAATTAATAAGGACCGGTTGAAAGCCGATTTAGATGACGTCAAAAAG
AAAATTCAGACGTTACTGGACACCTATTTTGTGTTTGATGAAGCAACCACCGCTAATATTCAACGTCAAGCTGGCCAATT
GGTTCAAAAATTAGTAAGTGTCACGTTAACTTTTAGAGCAGCCTTTCAAGCTGAAAAAGAACGGCGCCATTTATTGGACT
TTAGTGATTTAGAACAACTTTGTCTGACAATTTTATCGGTAGAGGATTCGCCGGCAAGGGCCTTTTACCAACAAAAATTT
AGCGAAGTGCTAGTTGATGAATACCAAGATACCAATCCGCTCCAAGAAACGATTATCCAAAAGGTCACTTCTGATCATCC
GCGCAATCTCTTCATGGTCGGGGATGTCAAGCAGTCAATTTATGCTTTCCGATTGGCCGATCCAAGCTTATTTAAGAATA
AGTATAACGAATTTGGTGTGACGGAAGATGAACGTGATAGTGAACGAATCATCTTAGCTGAAAACTTCCGTTCGCGCCGC
AATATCGATGACTTTACCAATCTGATTTTTAAACAATTGATGGACGAAAATCTCGGCGAGTTAGACTACGACGAAAATGC
GGCCTTACAATATGGTGCTCGTTATTATCCTGACCAGCACCCAACCGCGCCAACCGAACTCTTGCTTTATGAAACTAAGC
CGGAAGAGGCAGTTGCCAACCCGCAATTAGATAAGAGTGAGGGCCAAGTCGTGGCGGTCGCTAAACGGATTCAAGCCATG
ATGACTAATGGGGAACAGATTTGGGATAAGAAACTCGAAAAAATGCGGCCGATTGAATATCGGGACATCGTTTTACTAGC
CCCAACCCGTGGTAATAATTTATTTATCCTGGACTACTTCAAACGCTTCGGTTTGCCGGTTGTGATTAAGGATGCGCAAA
ATTATTTCCAAACGACTGAAGTCCAAATTATGTTAGCCCTACTTCAGGTGATTGATAATCCTAACCAAGATATTTCACTG
GTCAGTGTTTTACGCTCACCAATTGTCGGGTTAAACGAAAATGAATTGGCATTAATTCGGATTAACGATAAGACGGATGA
TTATTATCAAGCGGTCTTTAACTTTATTGACCAATATAATGAGCAGAAAGTCGGCCACTTAGGCCAACAAGTGATGCAGA
AGCTGACGCACTTCATGGCTTTATTGACGTCGTTTAGAACAATCGCTCGCCAACAACCGATTGTGGACTTAATTTGGACG
ATCTACCAAGAAACTGGCTTTTTAGATTATGTCGGTGGGATGCCAGCTGGACGTCAACGTCAAGCTAATTTACACGCGCT
TTATGAACGTGCCACGGCGTATGAAGAAAATGGCTTTAAAGGACTCTTCCAATTTATTCAATTTATTGAACGTCTCCAGA
AACAAGATAAGGATTTAGCACAACCAACGAGCTTAGAAAATCAGGACGCCATCTCTGTTATGACCATTCACGGGAGTAAG
GGGCTTGAATTTCCAGTCGTCTTTTTAATCGATACTAGTCGTCGCTTTAACCAACAGGATTTACAACGCTCATATGTTTT
GGATAATCATGGTGGTTTAGGGGTCGTTTATTTAGATAGCCAAAAGCGGTTGAAGGTGCCAACACTGCCAGAATTAGCGA
TTACCGCCCAGAAACGAAAAAAATTACGAGCAGAAGAAATGCGAAAACTATACGTTGCTTTGACACGTGCGGAACAACGG
TTAATCATCGTTGGCCATTGTGATAACCAAGCCCAATTGATGAAGCAATGGCAAAAAGGGCAAAATAGTACCCAACAAAT
ATTAGATGATGGTGTTCGCAACGATGCCGCAAGTTTACTGGACTGGATAGGTCTTAGCTTAATTCGCCATCCACAAGCTA
AAGATTGGACCACTGATTATGAGCCACTACTGGCTTTAAGTGGTGATCAAACCGAATTTAAGTTGATTATGACTAATGAA
ACCTTACTTGGCGAAATACCAGGTAGTCTGCATAACGATCAGGACTGGGGACAACAACAGCAAAAGGCACTTCAAACAAT
TGATTTAAACAGTGGGATAGCAACAGCGATTGCTGAGCAATTAACCTTTAAGTATCCCGCTAATGTTGCCACCCAAACGA
CGGCTTATCAATCTGTTTCGGAAGTCAAACGCCTTTTTGAAGACCCTGATAATACCGGATTGGGACAATTAGACCTAGCG
GCTGAACAACCGCGCCAAGCAAATCGCTACGTAACGGATAGTTTAGTACAACCACAGTTCTTACAAACTGTTCAACAACC
AACGGCAGCAGAAGTGGGGACCGCAACGCATTTGGTCTTGCAAGAAATTGATTTAACGCAACCAGTAACGCTAGCGAGTT
TGCAAGCCCTTGTCGATCGACTTGTTTTACAGAATAGTTTAAGCGCCAATGTTGCGGCTAAGATTAATCTTAATCACCTA
TTAACCTTCTTTGAAAGTGACTTGGGACAGCAATTATTAGCCCATCCAGAAGCGGTTAAGCGGGAAGTGCCATTTTCGTT
GTTATTACCAGCAGAAACGATTTTTGAAGGCATTCGTCCCACAGATGACGATCACGTCCTAATCCATGGGATTATGGATG
GTTATTTATTGACTGACGACGGTTGTTATTTATTTGATTACAAAACCGACTTTATTGACCCAAGTGATCAAGAAGCGGCG
ATTGAACGGGTTAAAACCCGCTATGCTGGCCAGATTAACCTCTATAGTGCTGCTTTAAACCAGATTGCCCAAAAGCCAGT
GACCCATAAATATCTCTATTTATTGTCAATTGGCGAGTTGGTTGAGATTAACTAG

Domains

Predicted by InterProScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (470-884)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1065-1227)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (7-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	37.165	100	0.379