Detailed information NTG0074276

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: K6K02_RS01610
• Genome accession: NZ_AP024626
• Coordinates: 293431..294552 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain BEST3125
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 288431..299552

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6K02_RS01585 (BsBEST3125_02660)	ycdA	288438..289457 (-)	1020	WP_049832648.1	DUF4352 domain-containing protein	-
K6K02_RS01590	-	289526..289657 (-)	132	WP_003246334.1	hypothetical protein	-
K6K02_RS01595 (BsBEST3125_02670)	ycdB	289831..291249 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
K6K02_RS01600 (BsBEST3125_02680)	ycdC	291436..292797 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
K6K02_RS01605 (BsBEST3125_02690)	cwlK	292805..293308 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
K6K02_RS01610 (BsBEST3125_02700)	rapF	293431..294552 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
K6K02_RS01615 (BsBEST3125_02710)	ycdF	294659..295435 (+)	777	WP_003246415.1	SDR family oxidoreductase	-
K6K02_RS01620 (BsBEST3125_02720)	ycdG	295460..297145 (+)	1686	WP_221255716.1	alpha-glucosidase	-
K6K02_RS01625 (BsBEST3125_02730)	znuA	297333..298292 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
K6K02_RS01630 (BsBEST3125_02740)	znuC	298348..299043 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=74276 K6K02_RS01610 WP_003246478.1 293431..294552(+) (rapF) [Bacillus subtilis strain BEST3125]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=74276 K6K02_RS01610 WP_003246478.1 293431..294552(+) (rapF) [Bacillus subtilis strain BEST3125]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAACCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405