Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   BsBEST3145_RS17855 Genome accession   NZ_AP024625
Coordinates   3400434..3402758 (-) Length   774 a.a.
NCBI ID   WP_003244180.1    Uniprot ID   O32215
Organism   Bacillus subtilis strain BEST3109     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3395434..3407758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BsBEST3145_RS17845 (BsBEST3109_34310) cysI 3396705..3398420 (-) 1716 WP_003243849.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -
  BsBEST3145_RS17850 (BsBEST3109_34320) cysJ 3398446..3400263 (-) 1818 WP_003243259.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  BsBEST3145_RS17855 (BsBEST3109_34330) helD 3400434..3402758 (-) 2325 WP_003244180.1 DNA helicase IV Machinery gene
  BsBEST3145_RS17860 (BsBEST3109_34340) yvgT 3402956..3403564 (-) 609 WP_003219999.1 trimeric intracellular cation channel family protein -
  BsBEST3145_RS17865 (BsBEST3109_34350) bdbC 3403751..3404167 (-) 417 WP_003228417.1 disulfide oxidoreductase -
  BsBEST3145_RS17870 (BsBEST3109_34360) bdbD 3404172..3404840 (-) 669 WP_003228414.1 disulfide bond formation protein BdbD -
  BsBEST3145_RS17875 (BsBEST3109_34370) cadA 3404960..3407068 (-) 2109 WP_014906546.1 heavy metal translocating P-type ATPase -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 89925.54 Da        Isoelectric Point: 6.5124

>NTDB_id=74242 BsBEST3145_RS17855 WP_003244180.1 3400434..3402758(-) (helD) [Bacillus subtilis strain BEST3109]
MNQQDKEWKEEQSRIDEVLKELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREH
NHRRMDQQLKRIHQLKKSPYFGRIDFIENGEEQAERIYIGLASCLDEKEEHFLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFMIKNGTLKAMFNTDMTIGDEMLQEVLSHHSDTQMKNIVSTIQKEQNQIIRNEKSKILIVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESPFDQLEYCLTE
TKGGDFPTRLAGITWKAGLSFQQFINEYVTRLSSEGMIFKNIIFRGQKLITKEQIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
TQLYLQLFSGWGGKFQHEKMDAIGELTRSAFTDNKLLYEDAAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTINGDQRMDACFEDEPAEYVRLKRTYRSTRQIVEFTKAMLQDGADIEPFNRSGEMPLVV
KTEGHESLCQKLAQEIGRLKKKGHETIAVICKTAHQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGEASPFVTAVPPHLYQIAE

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=74242 BsBEST3145_RS17855 WP_003244180.1 3400434..3402758(-) (helD) [Bacillus subtilis strain BEST3109]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGAAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCTAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAC
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCAATTGAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGAGCAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAGGAGGAACACTTTCTGA
TCTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCCCCAGGAAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATGATCAAAAACGGCACTCTCAAGGCGATGTTTAATACCGATAT
GACGATTGGCGATGAAATGCTGCAAGAGGTATTAAGCCATCACTCTGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGATCCTGATCGTGCAGGGAGCGGCTGGCAGCGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGCGGTGTCATTGATGCCGGACAAATCGTTTTGTTTTC
ACCCAACTTCTTATTTAACAGCTATGTCTCCTCTGTTTTGCCGGAGTTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCCCCTTTGATCAGCTCGAATATTGCCTGACCGAA
ACAAAGGGAGGCGATTTTCCAACAAGGCTTGCAGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTAATGA
ATACGTCACACGGCTTTCCTCTGAAGGGATGATTTTTAAAAATATTATATTCCGCGGACAAAAGCTGATCACAAAGGAGC
AAATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAATCGGATGGAGCAAACCGCGAAGTGGCTGCTG
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTTGTTCATGAAGCTGAGCTGCTTGACAAAGAGGA
CTACTTGGACGTGTACAAAAAACTGCAGGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAACAGGCAGTCAGGCTCCTTGCATTTCTTGATGTT
ACACAACTTTATTTGCAATTGTTTTCTGGCTGGGGAGGAAAATTTCAACATGAGAAGATGGATGCGATAGGTGAGCTGAC
CCGTTCGGCATTTACGGATAATAAATTGCTCTATGAGGACGCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAAATCAAACATCTATTTATTGACGAAGCCCAGGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGCATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCATAAACGGGGATCA
GCGTATGGATGCGTGCTTTGAGGATGAGCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAGTTTACCAAAGCGATGCTTCAAGATGGAGCGGATATTGAGCCGTTTAATAGAAGCGGGGAAATGCCGCTCGTTGTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGCCGGCTCAAGAAAAAAGGGCACGAAACAAT
AGCCGTCATCTGCAAGACTGCGCATCAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTTCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCGAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCGGTATTTTATACAGGTGAAGCCAGTCCGTTTGTAACCGCTGTGCCGCCTCACTTATATCAGATTGCTG
AATGA

Domains

Predicted by InterProScan.

AAA domain (AAA_19)

(217-580)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-751)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6VSX
  PDB 6WVK
  PDB 6ZCA
  PDB 6ZFB
  PDB 8XA8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment