Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QDW67_RS02635 Genome accession   NZ_CP122681
Coordinates   554520..555953 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain ETEC4068     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 549520..560953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDW67_RS02600 (QDW67_02600) elbB 550212..550865 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  QDW67_RS02605 (QDW67_02605) mtgA 550862..551590 (+) 729 WP_000047064.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QDW67_RS02610 (QDW67_02610) yrbL 551587..552219 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  QDW67_RS02615 (QDW67_02615) npr 552432..552704 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QDW67_RS02620 (QDW67_02620) rapZ 552701..553555 (-) 855 WP_000243741.1 RNase adapter RapZ -
  QDW67_RS02625 (QDW67_02625) ptsN 553601..554092 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QDW67_RS02630 (QDW67_02630) hpf 554210..554497 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QDW67_RS02635 (QDW67_02635) rpoN/rpoN1 554520..555953 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  QDW67_RS02640 (QDW67_02640) lptB 556001..556726 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QDW67_RS02645 (QDW67_02645) lptA 556733..557290 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QDW67_RS02650 (QDW67_02650) lptC 557259..557834 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  QDW67_RS02655 (QDW67_02655) kdsC 557831..558397 (-) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QDW67_RS02660 (QDW67_02660) kdsD 558418..559403 (-) 986 Protein_519 arabinose-5-phosphate isomerase KdsD -
  QDW67_RS02665 (QDW67_02665) yrbG 559417..560394 (-) 978 WP_000922881.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=742322 QDW67_RS02635 WP_000809051.1 554520..555953(-) (rpoN/rpoN1) [Escherichia coli strain ETEC4068]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=742322 QDW67_RS02635 WP_000809051.1 554520..555953(-) (rpoN/rpoN1) [Escherichia coli strain ETEC4068]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGATACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCATTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465