Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QDW51_RS04150 Genome accession   NZ_CP122604
Coordinates   867871..868851 (+) Length   326 a.a.
NCBI ID   WP_282832931.1    Uniprot ID   -
Organism   Escherichia coli strain ETEC6334     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 862871..873851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDW51_RS04120 (QDW51_04120) - 863248..864234 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  QDW51_RS04125 (QDW51_04125) hemW 864548..865684 (-) 1137 WP_063085753.1 radical SAM family heme chaperone HemW -
  QDW51_RS04130 (QDW51_04130) rdgB 865677..866270 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  QDW51_RS04135 (QDW51_04135) yggU 866278..866568 (-) 291 WP_001277223.1 DUF167 family protein YggU -
  QDW51_RS04140 (QDW51_04140) yggT 866565..867131 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  QDW51_RS04145 (QDW51_04145) yggS 867149..867853 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  QDW51_RS04150 (QDW51_04150) pilT 867871..868851 (+) 981 WP_282832931.1 type IV pilus twitching motility protein PilT Machinery gene
  QDW51_RS04155 (QDW51_04155) ruvX 868966..869382 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  QDW51_RS04160 (QDW51_04160) yqgE 869382..869945 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  QDW51_RS04165 (QDW51_04165) gshB 870054..871004 (-) 951 WP_000593273.1 glutathione synthase -
  QDW51_RS04170 (QDW51_04170) rsmE 871017..871748 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QDW51_RS04175 (QDW51_04175) endA 871828..872535 (-) 708 WP_000286500.1 deoxyribonuclease I -
  QDW51_RS04180 (QDW51_04180) yggI 872630..873127 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35948.08 Da        Isoelectric Point: 5.7980

>NTDB_id=741272 QDW51_RS04150 WP_282832931.1 867871..868851(+) (pilT) [Escherichia coli strain ETEC6334]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGASPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=741272 QDW51_RS04150 WP_282832931.1 867871..868851(+) (pilT) [Escherichia coli strain ETEC6334]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGTCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTGGGAGAGCTGCGTGACAGCGAAACGATACGTCTGGCGCTGACGG
CGGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362