Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QC806_RS02580 Genome accession   NZ_CP122507
Coordinates   533819..535252 (-) Length   477 a.a.
NCBI ID   WP_284631822.1    Uniprot ID   -
Organism   Escherichia coli strain W444     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528819..540252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QC806_RS02545 elbB 529510..530163 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  QC806_RS02550 mtgA 530160..530888 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QC806_RS02555 yrbL 530885..531517 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  QC806_RS02560 npr 531731..532003 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QC806_RS02565 rapZ 532000..532854 (-) 855 WP_000243741.1 RNase adapter RapZ -
  QC806_RS02570 ptsN 532900..533391 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QC806_RS02575 hpf 533509..533796 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QC806_RS02580 rpoN/rpoN1 533819..535252 (-) 1434 WP_284631822.1 RNA polymerase factor sigma-54 Machinery gene
  QC806_RS02585 lptB 535300..536025 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QC806_RS02590 lptA 536032..536589 (-) 558 WP_001723950.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QC806_RS02595 lptC 536558..537133 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  QC806_RS02600 kdsC 537130..537696 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QC806_RS02605 kdsD 537717..538703 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  QC806_RS02610 yrbG 538717..539694 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54019.81 Da        Isoelectric Point: 4.3663

>NTDB_id=740866 QC806_RS02580 WP_284631822.1 533819..535252(-) (rpoN/rpoN1) [Escherichia coli strain W444]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESISDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=740866 QC806_RS02580 WP_284631822.1 533819..535252(-) (rpoN/rpoN1) [Escherichia coli strain W444]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAAGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463