Detailed information NTG0740412

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QAO22_RS05485
• Genome accession: NZ_CP122490
• Coordinates: 1101031..1104684 (-)
• Length: 1217 a.a.
• NCBI ID: WP_000154918.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain GE-MSSA40
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1096031..1109684

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QAO22_RS05460 (QAO22_05440)	-	1096466..1096774 (-)	309	WP_000390575.1	metal-sulfur cluster assembly factor	-
QAO22_RS05465 (QAO22_05445)	-	1096888..1097712 (+)	825	WP_001182436.1	Cof-type HAD-IIB family hydrolase	-
QAO22_RS05470 (QAO22_05450)	-	1097764..1099080 (+)	1317	WP_001124516.1	CoA-disulfide reductase	-
QAO22_RS05475 (QAO22_05455)	-	1099251..1099640 (-)	390	WP_000902807.1	YisL family protein	-
QAO22_RS05480 (QAO22_05460)	-	1099965..1100867 (-)	903	WP_000670755.1	fumarylacetoacetate hydrolase family protein	-
QAO22_RS05485 (QAO22_05465)	addA	1101031..1104684 (-)	3654	WP_000154918.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QAO22_RS05490 (QAO22_05470)	addB	1104685..1108161 (-)	3477	WP_000172374.1	helicase-exonuclease AddAB subunit AddB	-
QAO22_RS05495 (QAO22_05475)	lepB	1108321..1108896 (-)	576	WP_000711894.1	signal peptidase I	-
QAO22_RS05500 (QAO22_05480)	lepB	1108912..1109436 (-)	525	WP_000758216.1	signal peptidase I	-

Sequence

Protein

• Length: 1217 a.a.
• Molecular weight: 141109.66 Da
• Isoelectric Point: 4.9678

>NTDB_id=740412 QAO22_RS05485 WP_000154918.1 1101031..1104684(-) (addA) [Staphylococcus aureus strain GE-MSSA40]
MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVERIIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRI
QEASIADPANAHLKNQRIKIHQAQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLTDLSKVFITAAYDALNKANDLFS
MMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGHEFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSD
YFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFQEILV
DEYQDTNRVQEKILSCIKTGAEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTINGDGTGRRIDLSQNFRSRKEVLS
TTNYIFKHMMDEQVGEVNYDESAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMK
TGSYRSATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQF
KEDELAQIRILSPNDDYFYQSIVNYINDEVADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIG
GRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKD
FNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLE
LEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEY
RSVNQLETMQNGNEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQ
GKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADAKKDIRMDEIMTFINSELYSIIAEAEQVYRELP
FVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILN
KEVKGYLYFFKFGTLQL

Nucleotide

• Length: 3654 bp

>NTDB_id=740412 QAO22_RS05485 WP_000154918.1 1101031..1104684(-) (addA) [Staphylococcus aureus strain GE-MSSA40]
ATGACAATTCCAGAGAAACCACAAGGCGTGATTTGGACTGACGCGCAATGGCAAAGTATTTACGCAACTGGACAAGATGT
ACTTGTTGCAGCCGCGGCAGGTTCAGGTAAAACAGCTGTACTAGTTGAGCGTATTATCCAAAAGATTTTACGTGATGGCA
TTGATGTCGATCGACTTTTAGTCGTAACGTTTACAAACTTAAGCGCACGTGAAATGAAGCATCGTGTAGACCAACGTATT
CAAGAGGCATCGATTGCTGATCCTGCAAATGCACACTTGAAAAACCAACGTATCAAAATTCATCAAGCACAAATATCTAC
ACTTCATAGTTTTTGCTTGAAATTAATTCAACAGCATTATGATGTATTAAATATTGACCCGAACTTTAGAACAAGCAGTG
AAGCTGAAAATATTTTATTATTAGAACAAACGATAGATGAGGTCATAGAACAACATTACGATATCCTTGATCCTGCTTTT
ATTGAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAG
CGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTA
TTCAACTACTAACAGACTTATCTAAAGTATTTATCACAGCTGCCTATGATGCTTTAAATAAGGCGAATGATTTGTTTAGT
ATGATGGATGGCGTCGATAAACATTTAGCTGTTATAGAAGATGAACGACGTTTAATGGGGCGTGTTTTAGAAGGTGGCTT
TATTGATATACCTTATTTAACTGGTCACGAATTTGGCGCGCGTTTGCCTAATGTAACAGCGAAAATTAAAGAAGCAAATG
AAATGATGGTCGATGCCTTAGAAGATGCTAAACTTCAGTATAAAAAATATAAATCATTAATTGATAAAGTGAAGAGTGAT
TACTTTTCAAGAGAAGCTGATGATTTGAAAGCTGATATGCAACAATTGGCGCCACGAGTAAAGTACCTTGCGCGTATTGT
GAAAGATGTTATGTCAGAATTCAATCGAAAAAAGCGTAGCAAAAATATTTTAGATTTTTCTGATTATGAACATTTTGCAT
TACAAATTTTAACTAATGAGGATGGTTCGCCTTCAGAAATTGCCGAATCATACCGTCAACACTTCCAAGAAATATTGGTC
GATGAGTATCAAGATACGAACCGAGTTCAAGAGAAAATACTATCTTGCATAAAAACGGGTGCAGAACATAATGGTAATTT
ATTTATGGTTGGAGATGTTAAGCAATCCATTTATAAATTTAGACAAGCTGATCCAAGTTTATTTATTGAAAAGTATCAAC
GCTTTACTATAAATGGAGATGGCACTGGACGTCGAATTGATTTGTCGCAAAACTTCCGTTCTCGAAAAGAAGTACTGTCA
ACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAATTATGATGAATCAGCACAATTGTATTA
TGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAA
CTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAA
ACAGGAAGCTATAGAAGTGCGACATACAAAGATATCGTTATTCTAGAACGCAGCTTTGGACAAGCTCGCAATTTACAACA
AGCCTTTAAAAATGAAGATATTCCATTCCATGTGAATAGTCGTGAAGGTTACTTTGAACAAACAGAAGTCCGCTTAGTAT
TATCATTTTTAAGAGCGATAGATAATCCATTACAAGATATTTATTTAGTTGGGTTAATGCGCTCCGTTATATATCAGTTC
AAAGAAGACGAATTAGCTCAAATTAGAATATTGAGTCCAAATGATGACTACTTCTATCAATCGATTGTAAATTACATTAA
TGACGAAGTAGCAGATGCTATTTTAGTTGATAAATTAAAGATGTTTTTATCAGATATTCAAAGTTACCAACAATATAGTA
AAGATCATCCGGTGTATCAGTTAATTGATAAATTTTATAATGATCATTATGTTATTCAATACTTTAGTGGACTTATTGGT
GGACGTGGAAGACGTGCAAACCTTTATGGTTTATTTAATAAAGCTATCGAGTTTGAGAATTCAAGTTTTAGAGGTTTATA
TCAATTTATTCGTTTTATCGATGAATTGATTGAAAGAGGCAAAGATTTTGGTGAGGAAAATGTAGTTGGTCCAAACGATA
ATGTCGTTAGAATGATGACAATTCATAGTAGTAAAGGTCTAGAGTTTCCATTTGTCATTTATTCTGGATTGTCAAAAGAT
TTTAATAAACGTGATTTGAAACAACCAGTTATTTTAAATCAGCAATTTGGTCTCGGAATGGATTATTTTGATGTGGATAA
AGAAATGGCATTTCCATCTTTAGCTTCGGTTGCATATAAAGCTGTTGCCGAAAAAGAACTTGTGTCAGAAGAAATGCGAT
TAGTCTATGTAGCATTAACAAGAGCGAAAGAACAACTTTATTTAATTGGTAGAGTGAAAAATGATAAATCGTTACTAGAA
CTAGAGCAATTGTCTATTTCTGGTGAGCATATTGCTGTCAATGAACGATTAACTTCACCAAATCCGTTCCATCTTATTTA
TAGTATTTTATCTAAACATCAATCTGCGTCAATTCCAGATGATTTAAAATTTGAAAAAGATATAGCACAAGTTGAAGATA
GTAGTCGTCCGAATGTAAATATTTCAATTATATACTTTGAAGATGTGTCTACAGAAACCATTTTAGATAATAATGAATAT
CGTTCGGTTAATCAATTAGAAACTATGCAAAATGGTAATGAGGATGTTAAAGCACAAATTAAACACCAACTTGATTATCA
ATATCCATATGTAAATGATACTAAAAAGCCATCAAAACAATCTGTTTCTGAATTGAAAAGGCAATATGAAACAGAAGAAA
GTGGCACAAGTTACGAACGAGTAAGACAATATCGTATCGGTTTTTCAACGTATGAACGACCTAAATTTCTAAGTGAACAA
GGTAAACGAAAAGCGAATGAAATTGGTACGTTAATGCATACAGTGATGCAACATTTACCATTCAAAAAAGAACGCATATC
TGAAGTTGAGTTACATCAGTATATCGATGGATTAATCGATAAACATATTATCGAAGCAGATGCGAAAAAAGATATCCGTA
TGGATGAAATAATGACATTTATCAATAGTGAGTTATATTCGATTATTGCTGAAGCAGAGCAAGTTTATCGTGAATTACCG
TTTGTAGTTAACCAAGCATTAGTTGACCAATTGCCACAAGGAGACGAAGACGTCTCAATTATTCAAGGTATGATTGACTT
AATCTTTGTTAAAGATGGTGTGCATTATTTTGTAGACTATAAAACCGATGCATTTAATCGTCGCCGTGGGATGACAGATG
AAGAAATTGGTACACAATTAAAAAATAAATATAAGATACAGATGAAATATTATCAAAATACGCTTCAAACGATTCTTAAT
AAAGAAGTTAAAGGTTATTTATACTTCTTCAAATTTGGTACATTGCAACTGTAG

Domains

Predicted by InterProScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (466-869)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (12-455)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1040-1210)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	36.963	100	0.382