Detailed information NTG0074035

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: AA2Tth_RS00095
• Genome accession: NZ_AP019792
• Coordinates: 18843..21509 (-)
• Length: 888 a.a.
• NCBI ID: WP_143584089.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain AA2-20
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13843..26509

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AA2Tth_RS00075 (TthAA220_00120)	-	14689..15321 (-)	633	WP_143584081.1	histidine phosphatase family protein	-
AA2Tth_RS00080 (TthAA220_00130)	purM	15318..16319 (-)	1002	WP_143584083.1	phosphoribosylformylglycinamidine cyclo-ligase	-
AA2Tth_RS00085 (TthAA220_00140)	gatB	16325..17734 (-)	1410	WP_143584085.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
AA2Tth_RS00090 (TthAA220_00150)	pilT	17745..18830 (-)	1086	WP_143584087.1	type IV pilus twitching motility protein PilT	Machinery gene
AA2Tth_RS00095 (TthAA220_00160)	pilF	18843..21509 (-)	2667	WP_143584089.1	type IV pilus assembly ATPase PilB	Machinery gene
AA2Tth_RS00100 (TthAA220_00170)	-	21506..21991 (-)	486	WP_014628818.1	YqeG family HAD IIIA-type phosphatase	-
AA2Tth_RS00105 (TthAA220_00180)	pgeF	22026..22754 (-)	729	WP_014628819.1	peptidoglycan editing factor PgeF	-
AA2Tth_RS00110 (TthAA220_00190)	surE	22751..23506 (-)	756	WP_014628820.1	5'/3'-nucleotidase SurE	-
AA2Tth_RS00115 (TthAA220_00200)	-	23565..23771 (-)	207	WP_143584091.1	cold-shock protein	-
AA2Tth_RS00120 (TthAA220_00210)	-	23926..25215 (+)	1290	WP_143584093.1	ABC transporter substrate-binding protein	-
AA2Tth_RS00125 (TthAA220_00220)	-	25267..26142 (+)	876	WP_143584095.1	sugar ABC transporter permease	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 97998.05 Da
• Isoelectric Point: 5.2761

>NTDB_id=74035 AA2Tth_RS00095 WP_143584089.1 18843..21509(-) (pilF) [Thermus thermophilus strain AA2-20]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLMLGEFLLKKGLISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAAALL
LRSDALRYGAVPIGFQNGEVEVVLSDPRHKEAVAQLLNRPARFYLALPQAWEELFRRAYPQKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRQGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYVDPEEDPP
DPGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNYEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAKKQEEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVIS
VVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKPY
GIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRD
SETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGLS
EAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTLE
EVLARTIE

Nucleotide

• Length: 2667 bp

>NTDB_id=74035 AA2Tth_RS00095 WP_143584089.1 18843..21509(-) (pilF) [Thermus thermophilus strain AA2-20]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAGAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTGGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTGTACGCCCTGGCCAAACACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAAAAGGACCTCATGCTCGGGGAGTTCCTCCTGAAAAAGGGGCTGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CTTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCCGACCCCTCCGCCGCCGCCCTCCTC
CTCCGCTCCGACGCCCTGCGTTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGGAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCACAAGGAGGCGGTGGCCCAGCTCCTCAATCGGCCCGCCCGCTTCTACCTCGCCCTCCCCCAGGCCTGGGAGGAGC
TCTTCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAGGCGCTA
AAGGAAGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCAGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGGAGGACCCCCCC
GATCCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCG
CCTCGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGA
ACTATGAGGTGGCCCCCGCTGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCAAAAAGCAGGAGGAGGAGGCCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGCCCA
GAAGTTCGTGAAGCAGGTGATCCGGGAGGCCTTCCTCCAGGACGCCTCCGACATCCACATTGAGCCCAGGCAGAACGACG
TCCAGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATCTCC
GTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAACGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGGGGC
CATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGGCCT
CGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAACCCTAC
GGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCC
CGACAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCGCAGG
CGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGAC
TCCGAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGCCGC
CCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCCCTTCAACATCTCCGCCGCCCTCATCGGCGTCCTCTCCCAGC
GCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTTTCC
GAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCGCTA
CGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCAAGG
AGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTCGAG
GAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (512-778)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	98.988	100	0.991
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	55.955	100	0.561