Detailed information NTG0740322

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QAO16_RS06240
• Genome accession: NZ_CP122489
• Coordinates: 1217054..1220707 (-)
• Length: 1217 a.a.
• NCBI ID: WP_000154949.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain GE-MSSA42
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1212054..1225707

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QAO16_RS06225 (QAO16_06215)	-	1213249..1214895 (-)	1647	WP_045178729.1	IS1182-like element ISSau3 family transposase	-
QAO16_RS06230 (QAO16_06220)	-	1215271..1215660 (-)	390	WP_000902813.1	YisL family protein	-
QAO16_RS06235 (QAO16_06225)	-	1215986..1216888 (-)	903	WP_000670753.1	fumarylacetoacetate hydrolase family protein	-
QAO16_RS06240 (QAO16_06230)	addA	1217054..1220707 (-)	3654	WP_000154949.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QAO16_RS06245 (QAO16_06235)	addB	1220708..1224184 (-)	3477	WP_000172367.1	helicase-exonuclease AddAB subunit AddB	-
QAO16_RS06250 (QAO16_06240)	lepB	1224344..1224919 (-)	576	WP_000711897.1	signal peptidase I	-
QAO16_RS06255 (QAO16_06245)	lepB	1224935..1225459 (-)	525	WP_000758217.1	signal peptidase I	-

Sequence

Protein

• Length: 1217 a.a.
• Molecular weight: 141287.81 Da
• Isoelectric Point: 4.9288

>NTDB_id=740322 QAO16_RS06240 WP_000154949.1 1217054..1220707(-) (addA) [Staphylococcus aureus strain GE-MSSA42]
MTIPEKPQGVIWTDAQWQSIYETGQDVLVAAAAGSGKTAVLVERIIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRI
QEASIADPANAHLKNQRIKIHQAQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLTDLSKVFITAAYDALNKAYDLFS
MMDGVDKHLAVIEDERRLMGRVLEGGFIDISYLTGHEFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSD
YFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFHEILV
DEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTLDGDGTGRRIDLSQNFRSRKEVLS
TTNYIFKHMMDEQVGEVRYDEAAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVFDMK
TGSYRSATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQF
KEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIG
GRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKD
FNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLE
LEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQIEDSSRPNVNISIIYFEDVSTETILDNDEY
RSVNQLETMQNGNEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQ
GKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADTKKDIRMDEIMTFINSELYSIIAEAEQVYRELP
FVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILN
KEVKGYLYFFKFGTLQL

Nucleotide

• Length: 3654 bp

>NTDB_id=740322 QAO16_RS06240 WP_000154949.1 1217054..1220707(-) (addA) [Staphylococcus aureus strain GE-MSSA42]
ATGACAATTCCAGAGAAACCACAAGGCGTGATTTGGACTGACGCGCAATGGCAAAGTATTTACGAAACTGGACAAGATGT
ACTTGTTGCAGCCGCGGCAGGTTCAGGTAAAACAGCTGTACTAGTTGAGCGTATTATCCAAAAGATTTTACGTGATGGCA
TTGATGTCGATCGACTTTTAGTCGTAACGTTCACAAACTTAAGTGCACGTGAAATGAAGCATCGTGTAGACCAACGTATT
CAAGAGGCATCGATTGCTGATCCTGCAAATGCACACTTGAAAAACCAACGCATCAAAATTCATCAAGCACAAATATCTAC
ACTTCATAGTTTTTGCTTGAAATTAATTCAACAGCATTATGATGTATTAAATATTGACCCGAACTTTAGAACAAGCAGTG
AAGCTGAAAATATTTTATTATTAGAACAAACGATAGATGAGGTCATAGAACAACATTACGATATCCTTGATCCTGCTTTT
ATTGAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAG
CGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTA
TTCAACTACTAACAGACTTATCTAAAGTATTTATCACAGCTGCCTATGATGCTTTAAATAAGGCGTATGATTTGTTTAGT
ATGATGGATGGCGTCGATAAACATTTAGCTGTTATAGAAGATGAACGACGTTTAATGGGGCGTGTTTTAGAAGGTGGCTT
TATTGATATATCTTATTTAACTGGTCACGAATTTGGTGCGCGTTTGCCTAATGTAACAGCGAAAATTAAAGAAGCAAATG
AAATGATGGTCGATGCCTTAGAAGATGCTAAACTTCAGTATAAAAAATATAAATCATTAATTGATAAAGTGAAAAGTGAT
TACTTTTCAAGAGAAGCTGATGATTTGAAAGCTGATATGCAACAATTGGCGCCACGAGTAAAGTACCTTGCGCGTATTGT
GAAAGATGTTATGTCAGAATTCAATCGAAAAAAGCGTAGCAAAAATATTCTGGATTTTTCTGATTATGAACATTTTGCAT
TACAAATTTTAACTAATGAGGATGGTTCGCCTTCAGAAATTGCCGAATCATACCGTCAACATTTTCATGAAATATTGGTC
GATGAGTATCAAGATACGAACCGAGTTCAAGAGAAAATACTATCTTGCATCAAAACGGGTGATGAACATAATGGTAATTT
ATTTATGGTTGGAGATGTTAAGCAGTCCATTTATAAATTTAGACAAGCTGATCCAAGTTTATTTATTGAAAAGTATCAAC
GGTTTACTTTAGATGGAGATGGCACTGGGCGTCGAATTGATTTGTCGCAAAACTTCCGTTCTCGTAAAGAAGTACTGTCA
ACGACAAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAGATATGATGAAGCAGCACAGTTGTATTA
TGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAA
CTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTTTGATATGAAA
ACAGGAAGCTATAGAAGTGCGACATACAAGGATATCGTTATTCTAGAACGCAGCTTTGGACAAGCTCGCAATTTACAACA
AGCCTTTAAAAATGAAGATATTCCATTCCATGTTAATAGTCGTGAAGGATACTTTGAACAAACAGAAGTCCGTTTAGTAT
TATCATTTTTAAGAGCGATAGATAATCCATTACAAGATATTTATTTAGTTGGGTTAATGCGTTCCGTTATTTATCAGTTC
AAAGAAGATGAATTAGCTCAAATTAGAATATTGAGTCCGAATGATGACTACTTCTATCAATCGATTGTAAATTACATTAA
TGACGAAGCAGCAGATGCTATTTTAGTTGATAAATTAAAAATGTTTTTATCAGATATTCAAAGTTACCAACAATATAGTA
AAGATCATCCGGTGTATCAGTTAATTGATAAATTTTATAATGATCATTATGTTATTCAATACTTTAGTGGACTTATTGGT
GGACGTGGACGACGTGCAAATCTTTATGGTTTATTTAATAAAGCTATCGAGTTTGAGAATTCAAGTTTTAGAGGTTTATA
TCAATTTATTCGTTTTATCGATGAATTGATTGAAAGAGGCAAAGATTTTGGTGAGGAAAATGTAGTTGGTCCAAACGATA
ATGTCGTTAGAATGATGACAATTCATAGTAGTAAAGGTCTAGAGTTTCCATTTGTCATTTATTCTGGATTGTCAAAAGAT
TTTAATAAACGTGATTTGAAACAACCAGTTATTTTAAATCAGCAATTTGGTCTCGGAATGGATTATTTTGATGTGGATAA
AGAAATGGCATTTCCATCTTTAGCTTCGGTTGCATATAAAGCTGTTGCCGAAAAAGAACTTGTGTCAGAAGAAATGCGAT
TAGTCTATGTAGCATTAACAAGAGCGAAAGAACAACTTTATTTAATTGGTAGAGTGAAAAATGATAAATCATTACTAGAA
CTAGAGCAATTGTCTATTTCTGGTGAGCACATTGCTGTCAATGAACGATTAACTTCACCAAATCCGTTCCATCTTATTTA
TAGTATTTTATCTAAACATCAATCTGCGTCAATTCCAGATGATTTAAAATTTGAAAAAGATATAGCACAAATTGAAGATA
GTAGTCGTCCGAATGTAAATATTTCAATTATATACTTTGAAGATGTGTCTACAGAAACCATTTTAGATAATGATGAATAT
CGTTCGGTTAATCAATTAGAAACTATGCAAAATGGTAATGAAGATGTTAAAGCACAAATTAAACACCAACTTGATTATCA
ATATCCATATGTAAATGATACTAAAAAGCCCTCAAAACAATCTGTTTCTGAATTGAAAAGACAATATGAAACTGAAGAAA
GTGGCACAAGTTACGAACGAGTAAGGCAATATCGTATCGGTTTTTCAACGTATGAACGACCTAAATTTCTAAGTGAACAA
GGTAAACGAAAAGCGAATGAAATTGGTACGTTAATGCATACAGTGATGCAACATTTACCATTCAAAAAAGAACGCATATC
TGAAGTTGAGTTACATCAGTATATCGATGGATTAATCGATAAACATATTATCGAAGCAGATACGAAAAAAGATATCCGTA
TGGATGAAATAATGACATTTATCAATAGTGAGTTATATTCGATTATTGCTGAAGCAGAGCAAGTTTATCGTGAATTACCG
TTTGTAGTTAACCAAGCATTAGTTGACCAATTGCCACAAGGAGACGAAGACGTCTCAATTATTCAAGGTATGATTGACTT
AATCTTTGTTAAAGATGGTGTGCATTATTTTGTAGACTATAAAACCGATGCATTTAATCGTCGCCGTGGGATGACAGATG
AAGAAATTGGTACACAATTAAAAAATAAATATAAGATACAGATGAAATATTATCAAAATACGCTTCAAACGATTCTTAAT
AAAGAAGTTAAAGGTTATTTATACTTCTTCAAATTTGGTACATTGCAACTGTAG

Domains

Predicted by InterProScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (466-869)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (12-455)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1040-1210)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	36.963	100	0.382