Detailed information NTG0740152

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH534_RS22300
• Genome accession: NZ_CP107463
• Coordinates: 4490475..4491275 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4485475..4496275

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH534_RS22265	gspH	4485631..4486146 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH534_RS22270	gspI	4486143..4486508 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH534_RS22275	gspJ	4486505..4487101 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH534_RS22280	gspK	4487098..4488084 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH534_RS22285	gspL	4488071..4489264 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH534_RS22290	-	4489257..4489742 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH534_RS22295	-	4489732..4490478 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH534_RS22300	pilD	4490475..4491275 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH534_RS22305	hrpB	4491323..4493752 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH534_RS22310	thpR	4493825..4494361 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH534_RS22315	sfsA	4494361..4495077 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH534_RS22320	dksA	4495240..4495695 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=740152 OH534_RS22300 WP_002888947.1 4490475..4491275(+) (pilD) [Klebsiella pneumoniae strain CRKP_12]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=740152 OH534_RS22300 WP_002888947.1 4490475..4491275(+) (pilD) [Klebsiella pneumoniae strain CRKP_12]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425