Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MKFW12EY_RS20275 Genome accession   NZ_AP019777
Coordinates   4497107..4498168 (-) Length   353 a.a.
NCBI ID   WP_064023024.1    Uniprot ID   -
Organism   Methylomonas koyamae strain Fw12E-Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4492107..4503168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKFW12EY_RS20255 (MKFW12EY_40330) pilQ 4493120..4495369 (-) 2250 WP_054758946.1 type IV pilus secretin family protein -
  MKFW12EY_RS20260 (MKFW12EY_40340) - 4495373..4495915 (-) 543 WP_221053652.1 pilus assembly protein PilP -
  MKFW12EY_RS20265 (MKFW12EY_40350) - 4495915..4496538 (-) 624 WP_064023022.1 type 4a pilus biogenesis protein PilO -
  MKFW12EY_RS20270 (MKFW12EY_40360) - 4496535..4497107 (-) 573 WP_054758945.1 PilN domain-containing protein -
  MKFW12EY_RS20275 (MKFW12EY_40370) pilM 4497107..4498168 (-) 1062 WP_064023024.1 pilus assembly protein PilM Machinery gene
  MKFW12EY_RS20280 (MKFW12EY_40380) - 4498411..4500726 (+) 2316 WP_245006365.1 penicillin-binding protein 1A -
  MKFW12EY_RS20285 (MKFW12EY_40390) rpmE 4500787..4500987 (-) 201 WP_054758943.1 50S ribosomal protein L31 -
  MKFW12EY_RS20290 (MKFW12EY_40400) - 4501097..4502434 (-) 1338 WP_280637603.1 MFS transporter -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37727.18 Da        Isoelectric Point: 4.7083

>NTDB_id=73956 MKFW12EY_RS20275 WP_064023024.1 4497107..4498168(-) (pilM) [Methylomonas koyamae strain Fw12E-Y]
MSWFSRNPSAVLGVDISTAAVKLLELSRAGARYKVESYAVVPLPQDAIVDKNITNVEQIGGAIKAAVKQSGTKAKHASVA
VAGSSVMTKIISMPASLSDAEMEEQIMVEADQYIPYSLDEVNLDFEVQGVTKSNPDMVDVLLAASRRENIEDRVAALAYA
GLKTVVVDVEAFAMENAFSLLVDQLPEGTGDMTVAIADIGATMTSLNILHAGKTVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYAADVLEPFKKAMLQQIARSLQFFVSSSANRGVDALILAGGCASIAGLDKLVERDLGIPSYIANPF
INMALSNRVKPQSLSNDTPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=73956 MKFW12EY_RS20275 WP_064023024.1 4497107..4498168(-) (pilM) [Methylomonas koyamae strain Fw12E-Y]
ATGAGCTGGTTCAGCAGGAATCCGTCGGCCGTGTTGGGTGTGGACATCAGTACTGCGGCGGTAAAGTTGTTGGAGCTAAG
TAGAGCCGGGGCGCGATACAAAGTCGAGAGTTACGCCGTCGTGCCGCTACCCCAGGATGCCATCGTCGACAAGAATATCA
CTAATGTCGAGCAAATTGGCGGAGCGATCAAGGCCGCAGTCAAACAGTCCGGCACTAAAGCCAAGCACGCCAGCGTCGCC
GTCGCCGGCTCTTCGGTGATGACGAAGATCATATCGATGCCGGCGTCGTTGTCCGATGCCGAGATGGAAGAACAGATTAT
GGTCGAAGCGGACCAATATATTCCGTATTCGCTGGACGAGGTAAATTTGGATTTCGAAGTCCAAGGCGTTACCAAAAGCA
ATCCGGACATGGTCGACGTATTGCTCGCCGCTTCCCGGCGGGAAAATATCGAAGACCGCGTTGCCGCGTTGGCCTATGCC
GGACTGAAGACTGTAGTGGTCGATGTCGAGGCATTCGCCATGGAAAACGCATTTTCGTTGCTGGTGGACCAGCTACCGGA
GGGGACCGGCGACATGACCGTGGCGATTGCCGATATCGGCGCCACGATGACCTCATTGAATATATTGCATGCCGGAAAGA
CGGTTTACACTCGCGAGCAGGGGTTCGGCGGCAAACAATTGACTGAGGAAATCCAGCGCCGCTACGGACTTTCCTACGAA
GAGGCGGGATTAGCCAAAAAACATGGCGGCTTGCCCGACAACTATGCGGCAGATGTGTTGGAACCGTTCAAAAAAGCCAT
GTTGCAGCAAATTGCCCGGTCATTGCAGTTTTTCGTTTCGTCCAGTGCCAACCGCGGCGTGGATGCGTTAATTCTCGCCG
GCGGCTGCGCATCTATCGCGGGTTTGGACAAATTGGTCGAACGCGATTTGGGCATACCGTCTTATATCGCCAATCCGTTT
ATCAATATGGCGCTGTCCAATCGGGTTAAGCCGCAAAGTCTCAGTAACGATACGCCGGCGATGATGATCGCTTGCGGCTT
GGCGTTAAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.977

100

0.521
  comM Acinetobacter nosocomialis M2

51.695

100

0.518
  comM Acinetobacter baylyi ADP1

49.575

100

0.496
  pilM Legionella pneumophila strain ERS1305867

42.151

97.45

0.411

Multiple sequence alignment