Detailed information NTG0739411

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH517_RS25560
• Genome accession: NZ_CP107297
• Coordinates: 5135447..5136247 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_44
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5130447..5141247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH517_RS25540	dksA	5131027..5131482 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
OH517_RS25545	sfsA	5131645..5132361 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH517_RS25550	thpR	5132361..5132897 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH517_RS25555	hrpB	5132970..5135399 (+)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH517_RS25560	pilD	5135447..5136247 (-)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH517_RS25565	-	5136244..5136990 (-)	747	WP_004145897.1	type II secretion system protein N	-
OH517_RS25570	-	5136980..5137465 (-)	486	WP_004151943.1	type II secretion system protein M	-
OH517_RS25575	gspL	5137458..5138651 (-)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH517_RS25580	gspK	5138638..5139624 (-)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH517_RS25585	gspJ	5139621..5140217 (-)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH517_RS25590	gspI	5140214..5140579 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH517_RS25595	gspH	5140576..5141091 (-)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=739411 OH517_RS25560 WP_002888947.1 5135447..5136247(-) (pilD) [Klebsiella pneumoniae strain CRKP_44]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=739411 OH517_RS25560 WP_002888947.1 5135447..5136247(-) (pilD) [Klebsiella pneumoniae strain CRKP_44]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425