Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OFU58_RS16540 Genome accession   NZ_CP107289
Coordinates   3397727..3398527 (-) Length   266 a.a.
NCBI ID   WP_213032058.1    Uniprot ID   -
Organism   Acinetobacter pittii strain BM4623     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3392727..3403527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OFU58_RS16520 (OFU58_16525) - 3393792..3394490 (-) 699 WP_014208343.1 tetratricopeptide repeat protein -
  OFU58_RS16525 (OFU58_16530) hisS 3394519..3395811 (-) 1293 WP_017385736.1 histidine--tRNA ligase -
  OFU58_RS16530 (OFU58_16535) ispG 3395808..3396923 (-) 1116 WP_086400274.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  OFU58_RS16535 (OFU58_16540) - 3396945..3397736 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  OFU58_RS16540 (OFU58_16545) pilF 3397727..3398527 (-) 801 WP_213032058.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OFU58_RS16545 (OFU58_16550) rlmN 3398542..3399777 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  OFU58_RS16550 (OFU58_16555) ndk 3399901..3400332 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  OFU58_RS16555 (OFU58_16560) iscX 3400446..3400646 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  OFU58_RS16560 (OFU58_16565) - 3400746..3401297 (-) 552 WP_348519940.1 phosphatase PAP2 family protein -
  OFU58_RS16565 (OFU58_16570) - 3401322..3402611 (-) 1290 WP_075383343.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30116.41 Da        Isoelectric Point: 9.0417

>NTDB_id=739349 OFU58_RS16540 WP_213032058.1 3397727..3398527(-) (pilF) [Acinetobacter pittii strain BM4623]
MSIPKLKTVLCMGIAVAFLASGCQTTHMQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=739349 OFU58_RS16540 WP_213032058.1 3397727..3398527(-) (pilF) [Acinetobacter pittii strain BM4623]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TATGCAAAAAAAAGACCCTGAGAAAGCGGTAAAAGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGGGACTTGG
ATTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTA
CTACAGCAAGAAGGTAGCAAACCTAATTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

75.769

97.744

0.741