Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N5937_RS16000 Genome accession   NZ_CP107262
Coordinates   3223652..3225085 (-) Length   477 a.a.
NCBI ID   WP_103177192.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain 2022CK-00320     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3218652..3230085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5937_RS15970 (N5937_15970) elbB 3220227..3220880 (+) 654 WP_263950228.1 isoprenoid biosynthesis glyoxalase ElbB -
  N5937_RS15975 (N5937_15975) mtgA 3220877..3221602 (+) 726 WP_159339888.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N5937_RS15980 (N5937_15980) npr 3221599..3221871 (-) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  N5937_RS15985 (N5937_15985) rapZ 3221868..3222722 (-) 855 WP_032614487.1 RNase adapter RapZ -
  N5937_RS15990 (N5937_15990) ptsN 3222768..3223259 (-) 492 WP_103177194.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N5937_RS15995 (N5937_15995) hpf 3223342..3223629 (-) 288 WP_103177193.1 ribosome hibernation promoting factor -
  N5937_RS16000 (N5937_16000) rpoN/rpoN1 3223652..3225085 (-) 1434 WP_103177192.1 RNA polymerase factor sigma-54 Machinery gene
  N5937_RS16005 (N5937_16005) lptB 3225133..3225858 (-) 726 WP_103177191.1 LPS export ABC transporter ATP-binding protein -
  N5937_RS16010 (N5937_16010) lptA 3225865..3226419 (-) 555 WP_103177190.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N5937_RS16015 (N5937_16015) lptC 3226388..3226963 (-) 576 WP_103177189.1 LPS export ABC transporter periplasmic protein LptC -
  N5937_RS16020 (N5937_16020) kdsC 3226960..3227526 (-) 567 WP_103824984.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N5937_RS16025 (N5937_16025) kdsD 3227541..3228527 (-) 987 WP_103177188.1 arabinose-5-phosphate isomerase KdsD -
  N5937_RS16030 (N5937_16030) - 3228541..3229518 (-) 978 WP_263950235.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54232.08 Da        Isoelectric Point: 4.3230

>NTDB_id=738841 N5937_RS16000 WP_103177192.1 3223652..3225085(-) (rpoN/rpoN1) [Leclercia adecarboxylata strain 2022CK-00320]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVETQQSQDNETLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLDD
ILESIGDDEIELEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIISEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=738841 N5937_RS16000 WP_103177192.1 3223652..3225085(-) (rpoN/rpoN1) [Leclercia adecarboxylata strain 2022CK-00320]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCTATTCGTCTGTTGCA
ACTGTCGACGCTGGAACTGCAGCAGGAGCTCCAGCAAGCGCTGGAAAGTAACCCTCTGCTCGAGCAAACCGATCTTCACG
ATGAGGTAGAAACGCAACAAAGCCAGGATAACGAGACCCTCGACACGGTGGATGCTCTTGAGCAAAAAGAGATGCCTGAC
GAGCTCCCTCTCGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGTACGCGCGCAGACTACCAGGATGA
CGAGCTGCCGGTCTATCAGGGCGAGACCACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACGCCGTTCT
CCGATACCGATCGCGCCATCGCCACCTCCATTGTGGATGCCGTGGATGAGACCGGCTATCTGACCGTGTCGCTGGACGAT
ATTCTGGAGAGCATAGGCGATGACGAGATTGAGCTTGAAGAGGTCGAAGCGGTCCTGAAACGCGTTCAGCGCTTTGACCC
GATTGGCGTTGCCGCGAGAGATCTCCGTGACTGCCTGCTCATTCAGCTTTCGCAGTTTGTTAAAGAGACCCCGTGGCTGG
AAGAGGCCCGGCTTATCATCAGCGAACATCTGGATCTGCTTGCCAACCACGATTTCCGCTCACTGATGCGCGTCACGCGG
CTGAAAGAGGAGATCCTGAAAGAGGCGGTCAACCTGATCCAGTCGCTGGATCCGCGCCCGGGGCAATCTATCCAGACCAG
CGAGCCGGAGTATGTTATTCCCGATGTGCTGGTGCGCAAACATAACGGTCTGTGGACCGTAGAGCTGAATTCCGACAGCA
TTCCCCGCCTGCAAATCAATCAGCAATACGCCTCAATGTGCACCAGCTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGTAATCTGCAGGAAGCCCGCTGGCTGATCAAAAGCCTTGAGAGCCGCAATGATACACTACTGCGCGTCAGCCGCTGCAT
CGTGGAGCAGCAACAGGCGTTTTTTGAGCAGGGTGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAGTCGACCATTTCTCGCGTGACGACGCAGAAGTATCTGCACAGTCCGCGTGGCATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCGCTGAGTGACAGTAAGTTGACCACCATGCTGTCCGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.525

100

0.491