Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   OD459_RS20690 Genome accession   NZ_CP107027
Coordinates   4068965..4070035 (+) Length   356 a.a.
NCBI ID   WP_048008264.1    Uniprot ID   A0AA46SIG2
Organism   Cytobacillus firmus strain M7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4063965..4075035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OD459_RS20650 (OD459_20650) - 4064188..4064361 (-) 174 WP_048008269.1 DUF2759 domain-containing protein -
  OD459_RS20655 (OD459_20655) - 4064487..4064966 (-) 480 WP_262173597.1 hypothetical protein -
  OD459_RS20660 (OD459_20660) - 4065054..4065689 (+) 636 WP_262173599.1 MBL fold metallo-hydrolase -
  OD459_RS20665 (OD459_20665) - 4065894..4066964 (+) 1071 WP_222498092.1 class I SAM-dependent methyltransferase -
  OD459_RS20670 (OD459_20670) - 4066999..4067244 (-) 246 WP_009332938.1 DUF2626 domain-containing protein -
  OD459_RS20675 (OD459_20675) - 4067398..4068096 (-) 699 WP_163143401.1 helix-turn-helix transcriptional regulator -
  OD459_RS20680 (OD459_20680) - 4068343..4068696 (+) 354 WP_048008265.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  OD459_RS20690 (OD459_20690) comGA 4068965..4070035 (+) 1071 WP_048008264.1 competence type IV pilus ATPase ComGA Machinery gene
  OD459_RS20695 (OD459_20695) comGB 4070022..4071053 (+) 1032 WP_262173602.1 competence type IV pilus assembly protein ComGB -
  OD459_RS20700 (OD459_20700) comGC 4071053..4071376 (+) 324 WP_252219385.1 competence type IV pilus major pilin ComGC -
  OD459_RS20705 (OD459_20705) comGD 4071373..4071813 (+) 441 WP_252219382.1 competence type IV pilus minor pilin ComGD -
  OD459_RS20710 (OD459_20710) - 4071797..4072126 (+) 330 WP_222497764.1 phosphatase -
  OD459_RS20715 (OD459_20715) comGF 4072140..4072556 (+) 417 WP_197247512.1 competence type IV pilus minor pilin ComGF -
  OD459_RS20720 (OD459_20720) comGG 4072546..4072929 (+) 384 WP_252219379.1 competence type IV pilus minor pilin ComGG -
  OD459_RS20725 (OD459_20725) - 4072942..4073439 (+) 498 WP_252219376.1 shikimate kinase -
  OD459_RS20730 (OD459_20730) - 4073518..4073703 (+) 186 WP_035329975.1 YqzE family protein -
  OD459_RS20735 (OD459_20735) - 4073747..4074541 (-) 795 WP_263599459.1 YqhG family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40081.29 Da        Isoelectric Point: 8.3347

>NTDB_id=737469 OD459_RS20690 WP_048008264.1 4068965..4070035(+) (comGA) [Cytobacillus firmus strain M7]
MSIIERLAERIIKDAVRSHASDIHIIPRRKDTLIQLRFGSQLTPRLYLPKEECDRLISHFKFTASMDIGEKRRPQSGAYS
LEVDGQMIGLRFSTLPSSHSESLVIRILPQQEQIPFYQISLFPDMTRKMLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHMFHRNVITLEDPIEKENDLVLQVQVNEKAGVSYSAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAEGAVYRLAEFGVNVLEIEQTLVAVTAQRLVELNCPYCEKECSPFCYGYGRWKRASVFELLAGRALNASIKKARGES
SLVKYRTLKEVITKGIALGYIKETEYSRWVHDDGEA

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=737469 OD459_RS20690 WP_048008264.1 4068965..4070035(+) (comGA) [Cytobacillus firmus strain M7]
TTGAGTATAATTGAAAGATTGGCAGAACGAATTATTAAAGACGCGGTCAGAAGCCATGCTTCCGACATCCACATCATTCC
CCGAAGAAAAGACACACTCATTCAGCTGAGGTTCGGCAGCCAGTTGACTCCAAGGCTTTATCTTCCCAAAGAAGAGTGTG
ACAGATTGATTTCCCATTTTAAATTTACAGCATCAATGGATATTGGTGAAAAAAGAAGGCCGCAGAGCGGTGCATACTCA
CTTGAAGTAGACGGACAAATGATTGGCCTGCGCTTTTCAACCCTCCCATCAAGCCACAGCGAGAGCCTCGTTATCAGAAT
TCTTCCCCAGCAGGAACAAATTCCTTTTTACCAAATCAGCCTTTTTCCAGACATGACAAGAAAAATGCTGGCACTGCTGA
AGCATGCTCATGGTTTAATTATTTTCACTGGCCCGACCGGCAGCGGTAAAACCACAACTTTGTATTCCCTTCTGAATGAA
ACCTCCCATATGTTCCATCGCAATGTCATCACCCTGGAAGATCCCATTGAAAAAGAGAATGACTTAGTTCTTCAAGTCCA
AGTGAATGAGAAAGCTGGCGTGTCTTATTCTGCAGGCTTAAAAGCAATCCTTCGCCATGACCCGGATATCATTATGGTCG
GTGAAATCCGGGATGCGGAAACGGCGAAGATTGCAGTTAGAGCAGCTCTCACCGGTCACCTTGTTTTAAGCACCATGCAT
ACACGGGATGCCGAGGGGGCTGTATACCGTTTAGCTGAGTTTGGAGTGAACGTGCTGGAAATTGAGCAGACTTTGGTGGC
GGTCACCGCACAGCGCCTCGTCGAATTGAATTGTCCATATTGTGAAAAGGAATGCTCCCCATTCTGCTATGGATATGGCA
GGTGGAAAAGGGCGAGTGTGTTTGAGCTGCTTGCAGGAAGGGCGCTCAATGCCTCCATAAAGAAGGCAAGAGGGGAAAGC
AGCCTTGTTAAGTATCGAACACTGAAGGAAGTCATAACTAAAGGGATTGCTCTGGGCTATATAAAGGAAACGGAGTACAG
CAGGTGGGTGCATGACGATGGAGAAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.337

100

0.553
  pilB Glaesserella parasuis strain SC1401

40

98.315

0.393