Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   OD459_RS14130 Genome accession   NZ_CP107027
Coordinates   2727465..2728844 (-) Length   459 a.a.
NCBI ID   WP_035332338.1    Uniprot ID   W7L2B7
Organism   Cytobacillus firmus strain M7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2722465..2733844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OD459_RS14110 (OD459_14110) ispF 2723649..2724134 (-) 486 WP_048011536.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  OD459_RS14115 (OD459_14115) ispD 2724149..2724835 (-) 687 WP_048011545.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  OD459_RS14120 (OD459_14120) - 2725082..2726170 (-) 1089 WP_048011538.1 PIN/TRAM domain-containing protein -
  OD459_RS14125 (OD459_14125) disA 2726389..2727462 (-) 1074 WP_048011539.1 DNA integrity scanning diadenylate cyclase DisA -
  OD459_RS14130 (OD459_14130) radA 2727465..2728844 (-) 1380 WP_035332338.1 DNA repair protein RadA Machinery gene
  OD459_RS14135 (OD459_14135) clpC 2729159..2731609 (-) 2451 WP_048011540.1 ATP-dependent protease ATP-binding subunit ClpC -
  OD459_RS14140 (OD459_14140) - 2731614..2732693 (-) 1080 WP_048011541.1 protein arginine kinase -
  OD459_RS14145 (OD459_14145) - 2732695..2733243 (-) 549 WP_048011542.1 UvrB/UvrC motif-containing protein -
  OD459_RS14150 (OD459_14150) - 2733267..2733728 (-) 462 WP_009336509.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49533.99 Da        Isoelectric Point: 7.4342

>NTDB_id=737446 OD459_RS14130 WP_035332338.1 2727465..2728844(-) (radA) [Cytobacillus firmus strain M7]
MAKRKTKFMCQECGYESAKWMGKCPGCGQWNTMVEEVEKPASTRRGAFAHSQGTAIAAKATPITSIETVSEPRIHTDLKE
LNRVLGGGIVRGSLVLIGGDPGIGKSTLLLQVSSQLANRKHSVLYISGEESMRQTKLRADRLGVSSDSLLVYSETSLEEI
SRTIETVNPDFVIIDSIQTIFHPEVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKELGLEEVANPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDKPTKATDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPANNLGGWSAPGGIELIGVSSVGEALKAALGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=737446 OD459_RS14130 WP_035332338.1 2727465..2728844(-) (radA) [Cytobacillus firmus strain M7]
ATGGCTAAAAGAAAAACGAAATTCATGTGCCAGGAATGCGGCTATGAATCAGCGAAATGGATGGGAAAATGTCCGGGGTG
CGGACAATGGAATACGATGGTGGAAGAGGTAGAGAAGCCAGCTTCAACGAGAAGAGGCGCTTTTGCACATTCACAGGGAA
CGGCCATAGCGGCAAAAGCTACGCCGATTACATCCATTGAAACAGTCAGCGAACCCCGCATACATACCGATTTAAAAGAA
TTAAACCGTGTCCTCGGGGGCGGGATTGTAAGAGGTTCCCTTGTTCTTATCGGTGGAGATCCGGGCATCGGGAAATCAAC
GCTTCTTCTTCAAGTGTCCTCACAGCTTGCGAACAGGAAGCATTCAGTCCTCTATATATCAGGGGAAGAATCCATGCGGC
AGACGAAGCTGCGGGCTGACCGTTTGGGTGTTTCTTCAGACAGTCTGCTCGTTTATTCGGAAACCAGCCTTGAAGAAATC
AGCAGAACGATTGAAACAGTCAACCCTGACTTTGTCATTATCGACTCCATACAGACGATTTTTCATCCGGAAGTGACATC
GGCGCCGGGGAGCGTTTCCCAGGTTCGGGAATGTACCGCTGAACTGATGCGCATTGGCAAAACGAAGGGAATTGCCATTT
TTATCGTAGGGCATGTTACAAAGGAGGGCTCCATTGCGGGTCCAAGACTTCTGGAGCACATGGTGGATACGGTTTTATAC
TTTGAAGGTGAACGCCATCATACATACCGGATTTTACGAGCTGTAAAAAACAGATTCGGGTCAACAAACGAGATGGGCAT
TTTTGAGATGAAGGAACTTGGGCTTGAAGAAGTAGCCAATCCATCTGAAATTTTCCTCGAAGAGCGGTCACAAGGGGCTG
CCGGTTCAACAGTTGTTGCTTCCATGGAGGGGACGAGGCCTGTTCTTGTTGAAATTCAGGCGCTTATTTCCCCGACAAGC
TTTGGAAATCCAAGACGAATGGCAACCGGGATCGATCATAACCGGGTCCCGCTCCTGATGGCCGTTTTGGAAAAACGGGT
CGGAATGCTCCTGCAAAATCAGGATGCCTACTTAAAAGTCGCTGGCGGAGTAAAGCTTGATGAACCGGCAATTGATTTGG
CCATAGCGGTCAGCATTGCGTCTAGCTTTCGGGATAAGCCGACAAAAGCAACAGACTGCATTATTGGAGAAGTAGGACTC
ACAGGCGAGGTCAGGAGAGTCTCTAGGATCGAACAAAGAGTGCAGGAGGCGGCCAAGCTGGGCTTTGAGCGGGTCATCCT
GCCGGCGAACAATCTTGGCGGCTGGAGCGCGCCGGGGGGCATCGAACTGATCGGAGTTAGCTCTGTTGGTGAAGCGCTAA
AGGCTGCGTTAGGGGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(66-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W7L2B7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.609

100

0.778
  radA Streptococcus pneumoniae Rx1

61.488

99.564

0.612
  radA Streptococcus pneumoniae D39

61.488

99.564

0.612
  radA Streptococcus pneumoniae R6

61.488

99.564

0.612
  radA Streptococcus pneumoniae TIGR4

61.488

99.564

0.612
  radA Streptococcus mitis NCTC 12261

61.488

99.564

0.612
  radA Streptococcus mitis SK321

61.488

99.564

0.612