Detailed information NTG0073741

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: ACRAD_RS02265
• Genome accession: NZ_AP019740
• Coordinates: 485753..486556 (+)
• Length: 267 a.a.
• NCBI ID: WP_005016505.1
• Uniprot ID: A0A2T1J2S1
• Organism: Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 480753..491556

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACRAD_RS02240 (ACRAD_04290)	-	481679..482956 (+)	1278	WP_005023382.1	glycosyltransferase family 1 protein	-
ACRAD_RS02245 (ACRAD_04300)	-	482985..483536 (+)	552	WP_005023384.1	phosphatase PAP2 family protein	-
ACRAD_RS02250 (ACRAD_04310)	iscX	483624..483824 (+)	201	WP_005023385.1	Fe-S cluster assembly protein IscX	-
ACRAD_RS02255 (ACRAD_04330)	ndk	483939..484370 (+)	432	WP_005016515.1	nucleoside-diphosphate kinase	-
ACRAD_RS02260 (ACRAD_04340)	rlmN	484493..485728 (+)	1236	WP_005023387.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
ACRAD_RS02265 (ACRAD_04350)	pilF	485753..486556 (+)	804	WP_005016505.1	type IV pilus biogenesis/stability protein PilW	Machinery gene
ACRAD_RS02270 (ACRAD_04360)	-	486547..487350 (+)	804	WP_005023389.1	helix-turn-helix domain-containing protein	-
ACRAD_RS02275 (ACRAD_04370)	ispG	487373..488488 (+)	1116	WP_005016500.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
ACRAD_RS02280 (ACRAD_04380)	hisS	488485..489780 (+)	1296	WP_005023391.1	histidine--tRNA ligase	-
ACRAD_RS02285 (ACRAD_04390)	-	489812..490516 (+)	705	WP_005023393.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 267 a.a.
• Molecular weight: 30279.57 Da
• Isoelectric Point: 9.5400

>NTDB_id=73741 ACRAD_RS02265 WP_005016505.1 485753..486556(+) (pilF) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
MRKPVYKTFVLLTVTLSALLVTACQTPDTLSKDPEKAVKVRTQLAAEYIKSGDLDAAKRALDQALEVDSRDATANMMMGV
LLQQEGSQLNLDKAESYFRRSISIEPKNAQARNNYGAYLYQIGRYKDAIEQLEIAGATLGYEQRYQALENLGRIYLKLGN
VANAEKTFKQALQANRDSSISMLELAEIFYLRQQIPAATQLYEQYVRRVGQKNQGARALWIGIRIARANDDKMGTQVLVN
QLRALFPDSQEYQRYLQLQHSTEAVWK

Nucleotide

• Length: 804 bp

>NTDB_id=73741 ACRAD_RS02265 WP_005016505.1 485753..486556(+) (pilF) [Acinetobacter radioresistens DSM 6976 = NBRC 102413 = CIP 103788 strain NBRC 102413]
TTGAGAAAGCCGGTGTATAAAACATTTGTATTACTTACAGTGACTTTGTCGGCCTTGCTTGTTACTGCTTGCCAGACTCC
TGATACCCTCAGTAAGGATCCAGAAAAGGCAGTCAAGGTTCGAACCCAGCTGGCAGCGGAATATATTAAATCGGGTGATC
TGGATGCTGCTAAACGGGCACTAGACCAGGCACTTGAAGTTGATTCCAGAGACGCCACTGCGAACATGATGATGGGAGTA
CTGCTCCAGCAAGAGGGGAGTCAGCTAAATCTGGACAAGGCTGAGAGTTATTTCAGACGCTCTATTTCAATTGAGCCTAA
AAATGCACAGGCGCGTAATAATTATGGCGCCTATTTGTACCAGATCGGTCGTTATAAAGATGCGATCGAACAACTGGAAA
TTGCTGGTGCAACTTTAGGCTATGAGCAGCGTTACCAGGCGCTTGAAAATCTGGGACGAATTTATCTTAAGCTCGGCAAT
GTTGCGAATGCTGAAAAAACATTCAAACAGGCATTGCAAGCTAACCGCGATTCTTCCATTTCAATGTTAGAGTTAGCGGA
AATTTTCTATTTGCGGCAACAGATTCCTGCTGCTACACAGCTTTATGAACAATATGTGCGTCGTGTAGGGCAAAAGAATC
AGGGTGCGCGTGCTCTTTGGATCGGAATTCGAATTGCGCGTGCCAATGATGATAAAATGGGAACACAAGTTTTGGTGAAC
CAGTTACGTGCCTTGTTCCCGGACAGCCAAGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_16) (44-82)

• Tetratricopeptide repeat (TPR_16) (150-208)

• Tetratricopeptide repeat (TPR_17) (98-130)

3D structure

• AlphaFold DB: A0A2T1J2S1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Acinetobacter baumannii D1279779	79.401	100	0.794
pilF	Acinetobacter baylyi ADP1	73.81	94.382	0.697