NTDB

Overview

Name	ruvB	Type	Machinery gene
Locus tag	P8R49_RS14135	Genome accession	NZ_CP121266
Coordinates	2607286..2608290 (-)	Length	334 a.a.
NCBI ID	WP_003229718.1	Uniprot ID	O32055
Organism	Bacillus subtilis strain 107105
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2602286..2613290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P8R49_RS14105 (P8R49_14105)	yrbG	2603260..2603916 (-)	657	WP_014480436.1	DUF421 domain-containing protein	-
P8R49_RS14110 (P8R49_14110)	-	2604114..2604503 (+)	390	WP_014477528.1	TIGR04086 family membrane protein	-
P8R49_RS14115 (P8R49_14115)	yajC	2604560..2604826 (-)	267	WP_004398708.1	preprotein translocase subunit YajC	-
P8R49_RS14120 (P8R49_14120)	tgt	2604863..2606008 (-)	1146	WP_003229725.1	tRNA guanosine(34) transglycosylase Tgt	-
P8R49_RS14125 (P8R49_14125)	queA	2606035..2607063 (-)	1029	WP_003229723.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
P8R49_RS14130 (P8R49_14130)	yrzS	2607093..2607293 (-)	201	WP_003222669.1	DUF2905 domain-containing protein	-
P8R49_RS14135 (P8R49_14135)	ruvB	2607286..2608290 (-)	1005	WP_003229718.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
P8R49_RS14140 (P8R49_14140)	ruvA	2608301..2608906 (-)	606	WP_003229717.1	Holliday junction branch migration protein RuvA	Machinery gene
P8R49_RS14145 (P8R49_14145)	bofC	2609045..2609557 (-)	513	WP_014480438.1	sporulation cell-cell signaling protein BofC	-
P8R49_RS14150 (P8R49_14150)	csbX	2609605..2610912 (-)	1308	WP_014480439.1	MFS transporter	-
P8R49_RS14155 (P8R49_14155)	iolG	2610983..2612008 (-)	1026	WP_014480440.1	inositol 2-dehydrogenase	-
P8R49_RS14160 (P8R49_14160)	yrzF	2612246..2612893 (+)	648	WP_014480441.1	phosphotransferase	-
P8R49_RS14165 (P8R49_14165)	-	2612939..2613061 (-)	123	WP_003229707.1	hypothetical protein	-
P8R49_RS14170 (P8R49_14170)	-	2613146..2613220 (+)	75	Protein_2740	hypothetical protein	-

Sequence

Protein

Download Length: 334 a.a. Molecular weight: 37467.78 Da Isoelectric Point: 5.1631

                                >NTDB_id=736766 P8R49_RS14135 WP_003229718.1 2607286..2608290(-) (ruvB) [Bacillus subtilis strain 107105]

MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE

LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT

RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI

TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP

AVYHHFQMEAPRYD

Nucleotide

Download Length: 1005 bp

                                >NTDB_id=736766 P8R49_RS14135 WP_003229718.1 2607286..2608290(-) (ruvB) [Bacillus subtilis strain 107105]

ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC

ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG

ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA

TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT

ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG

ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGCCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG

AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTAATGTCTCGCCTTGAGTACTATACACAAGAAGAACT

GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT

CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT

ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGTCTCGGTTTGGATCATATTGATCATAAGCTGCT

GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA

CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG

GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	100	100	1
ruvB	Streptococcus pneumoniae TIGR4	60.429	97.605	0.59
ruvB	Streptococcus pneumoniae R6	60.429	97.605	0.59
ruvB	Streptococcus pneumoniae D39	60.429	97.605	0.59
ruvB	Synechocystis sp. PCC 6803	57.143	98.503	0.563
ruvB	Helicobacter pylori 26695	52.744	98.204	0.518

Detailed information NTG0736766