Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   OAT02_RS09705 Genome accession   NZ_CP106947
Coordinates   1806219..1806653 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain 4F5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1801219..1811653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OAT02_RS09685 (OAT02_09685) - 1802133..1803296 (-) 1164 WP_053512462.1 aminotransferase A -
  OAT02_RS09690 (OAT02_09690) - 1803419..1803619 (-) 201 WP_000929277.1 hypothetical protein -
  OAT02_RS09695 (OAT02_09695) kinB 1803930..1805204 (+) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  OAT02_RS09700 (OAT02_09700) - 1805243..1805992 (-) 750 WP_000388471.1 DUF3967 domain-containing protein -
  OAT02_RS09705 (OAT02_09705) nucA/comI 1806219..1806653 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  OAT02_RS09710 (OAT02_09710) comJ 1806690..1807079 (+) 390 WP_000424030.1 competence protein ComJ -
  OAT02_RS09715 (OAT02_09715) metE 1807118..1809406 (-) 2289 WP_053512461.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  OAT02_RS09720 (OAT02_09720) - 1809918..1810475 (-) 558 WP_000062063.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=736758 OAT02_RS09705 WP_000811494.1 1806219..1806653(+) (nucA/comI) [Bacillus thuringiensis strain 4F5]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=736758 OAT02_RS09705 WP_000811494.1 1806219..1806653(+) (nucA/comI) [Bacillus thuringiensis strain 4F5]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549