Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   OAT02_RS08490 Genome accession   NZ_CP106947
Coordinates   1600244..1601287 (+) Length   347 a.a.
NCBI ID   WP_001013185.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain 4F5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1595244..1606287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OAT02_RS08455 (OAT02_08455) - 1595603..1596091 (+) 489 WP_000764261.1 hypothetical protein -
  OAT02_RS08460 (OAT02_08460) - 1596126..1596437 (-) 312 WP_001093237.1 hypothetical protein -
  OAT02_RS08465 (OAT02_08465) - 1596690..1596905 (+) 216 WP_001008326.1 DUF3912 family protein -
  OAT02_RS08470 (OAT02_08470) - 1596993..1597385 (+) 393 WP_000875390.1 hypothetical protein -
  OAT02_RS08475 (OAT02_08475) - 1597469..1598863 (-) 1395 WP_001094331.1 cation:dicarboxylase symporter family transporter -
  OAT02_RS08480 (OAT02_08480) - 1598970..1599212 (-) 243 WP_000440721.1 DUF2626 domain-containing protein -
  OAT02_RS08485 (OAT02_08485) - 1599343..1600038 (-) 696 WP_053512775.1 helix-turn-helix domain-containing protein -
  OAT02_RS08490 (OAT02_08490) comGA 1600244..1601287 (+) 1044 WP_001013185.1 competence type IV pilus ATPase ComGA Machinery gene
  OAT02_RS08495 (OAT02_08495) comGB 1601274..1602311 (+) 1038 WP_002101326.1 competence type IV pilus assembly protein ComGB -
  OAT02_RS08500 (OAT02_08500) comGC 1602323..1602622 (+) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  OAT02_RS08505 (OAT02_08505) comGD 1602619..1603074 (+) 456 WP_000813142.1 competence type IV pilus minor pilin ComGD -
  OAT02_RS08510 (OAT02_08510) - 1603067..1603369 (+) 303 WP_000829473.1 hypothetical protein -
  OAT02_RS08515 (OAT02_08515) comGF 1603339..1603809 (+) 471 WP_053512776.1 competence type IV pilus minor pilin ComGF -
  OAT02_RS08520 (OAT02_08520) comGG 1603806..1604177 (+) 372 WP_001231271.1 competence type IV pilus minor pilin ComGG -
  OAT02_RS08525 (OAT02_08525) - 1604354..1605004 (+) 651 WP_000183860.1 2OG-Fe(II) oxygenase -
  OAT02_RS08530 (OAT02_08530) - 1605120..1605617 (+) 498 WP_000836650.1 shikimate kinase -
  OAT02_RS08535 (OAT02_08535) - 1605656..1605856 (+) 201 WP_000106083.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39088.42 Da        Isoelectric Point: 8.3597

>NTDB_id=736755 OAT02_RS08490 WP_001013185.1 1600244..1601287(+) (comGA) [Bacillus thuringiensis strain 4F5]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTSQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGHELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=736755 OAT02_RS08490 WP_001013185.1 1600244..1601287(+) (comGA) [Bacillus thuringiensis strain 4F5]
ATGAATGGGATTGAAATTTTCGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGCCAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGCTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGACATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559