Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N6139_RS20055 Genome accession   NZ_CP106894
Coordinates   4126806..4128239 (+) Length   477 a.a.
NCBI ID   WP_058674772.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain EHX_PAT_001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4121806..4133239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N6139_RS20025 (N6139_20015) - 4122373..4123350 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  N6139_RS20030 (N6139_20020) kdsD 4123364..4124350 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  N6139_RS20035 (N6139_20025) kdsC 4124365..4124931 (+) 567 WP_003861856.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N6139_RS20040 (N6139_20030) lptC 4124928..4125503 (+) 576 WP_003861858.1 LPS export ABC transporter periplasmic protein LptC -
  N6139_RS20045 (N6139_20035) lptA 4125472..4126026 (+) 555 WP_003861860.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N6139_RS20050 (N6139_20040) lptB 4126033..4126758 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  N6139_RS20055 (N6139_20045) rpoN/rpoN1 4126806..4128239 (+) 1434 WP_058674772.1 RNA polymerase factor sigma-54 Machinery gene
  N6139_RS20060 (N6139_20050) hpf 4128262..4128549 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  N6139_RS20065 (N6139_20055) ptsN 4128632..4129123 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N6139_RS20070 (N6139_20060) rapZ 4129169..4130023 (+) 855 WP_003861870.1 RNase adapter RapZ -
  N6139_RS20075 (N6139_20065) npr 4130020..4130292 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  N6139_RS20080 (N6139_20070) mtgA 4130332..4131057 (-) 726 WP_032660497.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N6139_RS20085 (N6139_20075) elbB 4131054..4131707 (-) 654 WP_003861876.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54041.92 Da        Isoelectric Point: 4.3524

>NTDB_id=736517 N6139_RS20055 WP_058674772.1 4126806..4128239(+) (rpoN/rpoN1) [Enterobacter hormaechei strain EHX_PAT_001]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDAIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=736517 N6139_RS20055 WP_058674772.1 4126806..4128239(+) (rpoN/rpoN1) [Enterobacter hormaechei strain EHX_PAT_001]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTCCAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAGACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCACTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGCAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAATCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGTGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCTAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAACCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAGTCGATTCAGACCAG
CGAACCGGAATATGTCATTCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATAGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.743

100

0.484