Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   P6H83_RS20025 Genome accession   NZ_CP121199
Coordinates   4135953..4137335 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain E50     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4130953..4142335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P6H83_RS20010 (P6H83_20010) ettA 4132726..4134393 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  P6H83_RS20015 (P6H83_20015) nadS 4134553..4134666 (-) 114 Protein_3921 NadS family protein -
  P6H83_RS20020 (P6H83_20020) nadR 4134700..4135932 (-) 1233 WP_000093812.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  P6H83_RS20025 (P6H83_20025) radA/sms 4135953..4137335 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  P6H83_RS20030 (P6H83_20030) serB 4137384..4138352 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  P6H83_RS20035 (P6H83_20035) ytjB 4138458..4139102 (+) 645 WP_000124604.1 YtjB family periplasmic protein -
  P6H83_RS20040 (P6H83_20040) lplA 4139130..4140146 (+) 1017 WP_000105873.1 lipoate--protein ligase LplA -
  P6H83_RS20045 (P6H83_20045) - 4140178..4140441 (+) 264 WP_000566145.1 helix-turn-helix transcriptional regulator -
  P6H83_RS20050 (P6H83_20050) deoD 4140602..4141321 (-) 720 WP_000224879.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=736360 P6H83_RS20025 WP_001029698.1 4135953..4137335(-) (radA/sms) [Escherichia coli strain E50]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=736360 P6H83_RS20025 WP_001029698.1 4135953..4137335(-) (radA/sms) [Escherichia coli strain E50]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGAGTGGCAAAAGTCCAGAAACTCTCCGACATCAGCCTTGAGGAGCTGCCGCGTTTTTCTACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGGGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGTGCGGG
GAAATCCACGCTGCTGTTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCAATGACCGAACAGGGTCTGCGTGAAGTCAGCAACCCTTCGGCAATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCTGGTAGCTCAGTGATGGTAGTATGGGAAGGAACGCGTCCGCTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTCGCAGTAGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCG
TCACGGTGGTTTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43