Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   K6J89_RS13780 Genome accession   NZ_AP024621
Coordinates   2631687..2632097 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain BEST3095     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2626687..2637097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J89_RS13750 - 2626774..2626914 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  K6J89_RS13755 (BsBEST3095_26300) yqeF 2627220..2627951 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  K6J89_RS13760 (BsBEST3095_26310) cwlH 2628203..2628955 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  K6J89_RS13765 (BsBEST3095_26320) yqeD 2629142..2629768 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  K6J89_RS13770 (BsBEST3095_26330) gnd 2629787..2630680 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  K6J89_RS13775 (BsBEST3095_26340) yqeB 2630932..2631654 (+) 723 WP_010886572.1 hypothetical protein -
  K6J89_RS13780 (BsBEST3095_26350) nucA/comI 2631687..2632097 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  K6J89_RS13785 (BsBEST3095_26360) - 2632293..2632643 (+) 351 Protein_2679 sigma-70 family RNA polymerase sigma factor -
  K6J89_RS13790 (BsBEST3095_26370) spoIVCA 2632671..2634131 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  K6J89_RS22030 (BsBEST3095_26380) - 2634089..2634267 (-) 179 Protein_2681 hypothetical protein -
  K6J89_RS13795 (BsBEST3095_26390) arsC 2634622..2635041 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  K6J89_RS13800 (BsBEST3095_26400) acr3 2635053..2636093 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  K6J89_RS13805 (BsBEST3095_26410) arsK 2636116..2636556 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  K6J89_RS13810 (BsBEST3095_26420) arsR 2636617..2636934 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=73586 K6J89_RS13780 WP_009967785.1 2631687..2632097(-) (nucA/comI) [Bacillus subtilis strain BEST3095]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=73586 K6J89_RS13780 WP_009967785.1 2631687..2632097(-) (nucA/comI) [Bacillus subtilis strain BEST3095]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment