Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   P4B18_RS08640 Genome accession   NZ_CP120705
Coordinates   1773815..1775308 (-) Length   497 a.a.
NCBI ID   WP_124164313.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY11084     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1768815..1780308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4B18_RS08605 (P4B18_08610) - 1769666..1770115 (+) 450 WP_003094369.1 hypothetical protein -
  P4B18_RS08610 (P4B18_08615) - 1770128..1770739 (+) 612 WP_003098862.1 superoxide dismutase -
  P4B18_RS08615 (P4B18_08620) - 1770758..1771690 (+) 933 WP_003120918.1 ZIP family metal transporter -
  P4B18_RS08620 (P4B18_08625) - 1771787..1772059 (-) 273 WP_003094362.1 HPr family phosphocarrier protein -
  P4B18_RS08625 (P4B18_08630) rapZ 1772089..1772949 (-) 861 WP_003094361.1 RNase adapter RapZ -
  P4B18_RS08630 (P4B18_08635) ptsN 1772951..1773415 (-) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  P4B18_RS08635 (P4B18_08640) hpf 1773429..1773737 (-) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  P4B18_RS08640 (P4B18_08645) rpoN/rpoN1 1773815..1775308 (-) 1494 WP_124164313.1 RNA polymerase factor sigma-54 Machinery gene
  P4B18_RS08645 (P4B18_08650) lptB 1775534..1776259 (-) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  P4B18_RS08650 (P4B18_08655) lptA 1776259..1776786 (-) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  P4B18_RS08655 (P4B18_08660) lptC 1776773..1777345 (-) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  P4B18_RS08660 (P4B18_08665) - 1777354..1777893 (-) 540 WP_003098849.1 HAD family hydrolase -
  P4B18_RS08665 (P4B18_08670) kdsD 1777893..1778873 (-) 981 WP_003134758.1 arabinose-5-phosphate isomerase KdsD -
  P4B18_RS08670 (P4B18_08675) - 1779287..1780096 (+) 810 WP_003094343.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56068.24 Da        Isoelectric Point: 4.4327

>NTDB_id=734077 P4B18_RS08640 WP_124164313.1 1773815..1775308(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain NY11084]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDIELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=734077 P4B18_RS08640 WP_124164313.1 1773815..1775308(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain NY11084]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACATCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCGAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.457

100

0.475