Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N8S79_RS09735 Genome accession   NZ_CP106678
Coordinates   2038713..2040170 (+) Length   485 a.a.
NCBI ID   WP_046850031.1    Uniprot ID   A0A0F7KFP4
Organism   Nitrosomonas sp. PLL12-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2033713..2045170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8S79_RS09710 - 2034401..2035153 (-) 753 WP_046850027.1 endonuclease/exonuclease/phosphatase family protein -
  N8S79_RS09715 nadA 2035221..2036321 (-) 1101 WP_046850028.1 quinolinate synthase NadA -
  N8S79_RS09720 lptC 2036763..2037347 (+) 585 WP_235264344.1 LPS export ABC transporter periplasmic protein LptC -
  N8S79_RS09725 lptA 2037344..2037898 (+) 555 WP_046850030.1 lipopolysaccharide transport periplasmic protein LptA -
  N8S79_RS09730 lptB 2037936..2038658 (+) 723 WP_046851647.1 LPS export ABC transporter ATP-binding protein -
  N8S79_RS09735 rpoN/rpoN1 2038713..2040170 (+) 1458 WP_046850031.1 RNA polymerase factor sigma-54 Machinery gene
  N8S79_RS09740 - 2040350..2041048 (-) 699 WP_046850032.1 hypothetical protein -
  N8S79_RS09745 - 2042061..2043533 (+) 1473 WP_046851564.1 ISNCY-like element ISNco1 family transposase -
  N8S79_RS09750 - 2043813..2044211 (+) 399 WP_046850033.1 hypothetical protein -
  N8S79_RS09755 - 2044476..2045060 (-) 585 WP_046850034.1 DUF1353 domain-containing protein -

Sequence

Protein


Download         Length: 485 a.a.        Molecular weight: 55378.45 Da        Isoelectric Point: 5.2567

>NTDB_id=734071 N8S79_RS09735 WP_046850031.1 2038713..2040170(+) (rpoN/rpoN1) [Nitrosomonas sp. PLL12-2]
MKPTLHLKLTTSLKLTPQLQQSIKLLQLSTIELNQEIERMLQNNPLLELSDKTDYDQMHAEDFSLSLPLDRTSPIELNDA
QHQEKDDELSLPSSEETYRLGDYEIFSSHRESEEDEWDFTQQMINPPTLREHLIMQISLSQISERDRQIVRILIESLDDD
GYLAQDLQELFEMLPAELGIHLADLQTALKYLQQLDPPGLGARNLQECLLLQLQALPEDTPCRQEAILLVEHDLEILASK
DFKQIKKLLHCDDECLRSVQQLITQLNPKPGSTFNSAASRYVIPDVIVTKLNGAWVAKLNPESIPRLSINQLYAGILKRD
RNDSTQMLMSQLNEAKWMLKNIQQRSETIFRVSTIIVEHQQAFFEHGAVAMRPLVMREIAEALNLHESTVSRVTTQKFMH
TPQGIFELKYFFGSHVATHGGGACSAIAIRTLIKQLIQGEEQKKPLTDNRISQILAEQGIIVARRTVAKYRESMQIPPAN
LRKGL

Nucleotide


Download         Length: 1458 bp        

>NTDB_id=734071 N8S79_RS09735 WP_046850031.1 2038713..2040170(+) (rpoN/rpoN1) [Nitrosomonas sp. PLL12-2]
ATGAAACCTACTCTTCATCTTAAATTAACTACCAGTCTTAAGCTTACGCCACAGTTGCAACAGTCCATAAAATTGCTGCA
GCTTTCTACCATTGAGTTAAATCAGGAAATAGAACGTATGCTGCAGAATAATCCTCTGCTGGAATTAAGCGATAAGACGG
ATTATGATCAGATGCATGCAGAGGATTTCTCGCTCTCATTACCCTTGGATAGGACTTCGCCCATTGAGTTGAATGACGCA
CAACATCAAGAGAAGGATGATGAATTGAGCTTACCTTCCAGTGAAGAGACTTACAGGCTTGGTGATTATGAAATCTTTTC
CAGTCATCGAGAAAGTGAGGAGGATGAATGGGATTTTACCCAACAGATGATCAATCCTCCCACTTTGCGTGAACATCTCA
TTATGCAAATCAGCTTGAGCCAGATCAGCGAACGCGACCGTCAAATCGTGAGGATATTAATCGAAAGTCTGGATGATGAT
GGTTACCTTGCGCAGGACCTGCAAGAATTATTTGAAATGCTGCCAGCAGAGTTGGGCATTCATCTGGCTGACTTGCAGAC
AGCGCTTAAATATTTGCAACAATTGGATCCACCCGGTTTAGGTGCACGTAATTTGCAAGAGTGTCTACTCTTGCAGCTAC
AAGCACTGCCTGAAGACACGCCTTGTCGTCAGGAGGCGATTTTGCTTGTGGAGCACGATCTAGAGATACTTGCTTCAAAA
GATTTTAAACAGATTAAGAAGTTGTTGCATTGTGATGATGAATGCCTGCGTTCCGTGCAGCAACTTATTACTCAATTGAA
TCCCAAACCGGGTAGTACGTTCAATTCAGCTGCCTCGCGTTATGTCATACCTGACGTAATTGTGACTAAGCTTAATGGGG
CATGGGTAGCAAAACTTAATCCTGAATCAATTCCCCGCCTTAGTATTAATCAACTTTATGCTGGTATATTAAAGCGTGAT
CGAAATGATTCGACTCAAATGTTAATGAGTCAGCTTAATGAAGCCAAATGGATGCTTAAGAATATCCAGCAACGTTCTGA
AACTATTTTTCGAGTATCCACTATTATAGTAGAGCACCAGCAGGCTTTCTTCGAGCATGGGGCAGTTGCAATGCGTCCGC
TTGTCATGAGGGAAATTGCAGAAGCTCTGAATTTGCATGAATCTACCGTCTCACGTGTTACCACGCAAAAGTTTATGCAT
ACACCCCAAGGCATATTTGAGCTCAAGTATTTTTTTGGAAGCCATGTCGCAACCCATGGTGGCGGAGCCTGCTCTGCTAT
TGCAATTCGTACTTTAATCAAACAATTGATACAAGGTGAGGAACAGAAAAAGCCGCTTACTGATAATCGGATTTCCCAGA
TTCTGGCTGAGCAGGGAATTATTGTTGCACGTCGTACGGTTGCAAAATACCGCGAATCGATGCAAATTCCCCCAGCTAAT
CTTCGTAAAGGGCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(119-313)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-483)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F7KFP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.116

100

0.515