Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   N7988_RS25805 Genome accession   NZ_CP106675
Coordinates   5006412..5007761 (-) Length   449 a.a.
NCBI ID   WP_000879428.1    Uniprot ID   W8YBC3
Organism   Bacillus cereus strain SRCM116293     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5001412..5012761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7988_RS25770 (N7988_25770) - 5001449..5002144 (-) 696 WP_000466510.1 hypothetical protein -
  N7988_RS25775 (N7988_25775) - 5002131..5002919 (-) 789 WP_098348325.1 hypothetical protein -
  N7988_RS25780 (N7988_25780) - 5002947..5003517 (-) 571 Protein_5014 hypothetical protein -
  N7988_RS25785 (N7988_25785) - 5003635..5004114 (-) 480 WP_098348324.1 hypothetical protein -
  N7988_RS25790 (N7988_25790) raiA 5004518..5005060 (-) 543 WP_000671186.1 ribosome-associated translation inhibitor RaiA -
  N7988_RS25795 (N7988_25795) cspC 5005384..5005581 (-) 198 WP_001990088.1 cold shock protein CspC -
  N7988_RS25800 (N7988_25800) - 5005708..5006412 (-) 705 WP_262314126.1 ComF family protein -
  N7988_RS25805 (N7988_25805) comFA 5006412..5007761 (-) 1350 WP_000879428.1 ATP-dependent helicase ComFA Machinery gene
  N7988_RS25810 (N7988_25810) - 5007889..5009319 (-) 1431 WP_000499471.1 C40 family peptidase -
  N7988_RS25815 (N7988_25815) - 5009468..5009782 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  N7988_RS25820 (N7988_25820) - 5009955..5010797 (-) 843 WP_098331218.1 DegV family protein -
  N7988_RS25825 (N7988_25825) - 5011034..5011669 (+) 636 WP_098331208.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51010.89 Da        Isoelectric Point: 9.9712

>NTDB_id=734052 N7988_RS25805 WP_000879428.1 5006412..5007761(-) (comFA) [Bacillus cereus strain SRCM116293]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=734052 N7988_RS25805 WP_000879428.1 5006412..5007761(-) (comFA) [Bacillus cereus strain SRCM116293]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAAAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCGTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8YBC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.357

98.441

0.506
  comFA Latilactobacillus sakei subsp. sakei 23K

41.439

89.755

0.372