Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   N7921_RS06025 Genome accession   NZ_CP106652
Coordinates   1157678..1161382 (+) Length   1234 a.a.
NCBI ID   WP_144485569.1    Uniprot ID   -
Organism   Bacillus safensis strain 33.4     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1152678..1166382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7921_RS06010 (N7921_06010) - 1152983..1153393 (+) 411 WP_025093362.1 DoxX family protein -
  N7921_RS06015 (N7921_06015) - 1153591..1154037 (-) 447 WP_048238419.1 ferritin-like domain-containing protein -
  N7921_RS06020 (N7921_06020) addB 1154191..1157700 (+) 3510 WP_263640136.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  N7921_RS06025 (N7921_06025) addA 1157678..1161382 (+) 3705 WP_144485569.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  N7921_RS06030 (N7921_06030) sbcD 1161409..1162584 (+) 1176 WP_144470097.1 exonuclease subunit SbcD -
  N7921_RS06035 (N7921_06035) - 1162581..1165976 (+) 3396 WP_144485567.1 AAA family ATPase -
  N7921_RS06040 (N7921_06040) - 1165996..1166295 (+) 300 WP_048238424.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142293.62 Da        Isoelectric Point: 5.1740

>NTDB_id=733702 N7921_RS06025 WP_144485569.1 1157678..1161382(+) (addA) [Bacillus safensis strain 33.4]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDIDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGQHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFKPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRKLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLEQFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLIPDFERYQSKTYLDFIGPALIRHQAMSSILEETGNVVLSHPSVFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
TTLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLPSDVLYDDSRIQQLLDSLQQRDLLTNEQVQSIDQEGITAFFSTSIGQ
KLQKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLKAAAPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=733702 N7921_RS06025 WP_144485569.1 1157678..1161382(+) (addA) [Bacillus safensis strain 33.4]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAACATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCCAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCACTCATGAATCGTGCTAATATTTC
TACCTTGCACTCTTTTTGTTTACAGGTGCTGCGTACCTTTTACTATGAGATTGACCTTGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGTCATGATATAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTATCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCT
CTCGCTCTATCAAAAGAACCGGGCGGCCCAGCACCGAGAGCGGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
GATCAGGCATCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCTACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTCAAA
GATGAATACTTTATGCGAAGTCCCGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCAGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGATTTGAAAGAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCGGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAAGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAAGGGCA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTAAACCAGATGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGTAAAGATACAACGACAGAAATGCTCCTTGTTCATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGGGAAGAACTAGAGACCGTACAGCTAGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTAGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAAATTGCAGTATCGGGACAT
TGTGATTTTGCTTCGCTCCATGCCGTGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGGATTCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCTATTGTTCATTTAGACGAAAATGAAATGGCACTGATCCGCACGAGTGA
TAAGAAAGGCACTTATTATGATGCTGTGAAAGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACAATTTTTCAGGTTGCTGCGTAAGTGGCGTGATTTCTCCATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAACCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCCTTTAGAGGGCTGTTCCGTTTTCTTCGCTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGATTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGACTGGCTGAT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTTATCCGTCACCAAGCGATGTCAT
CTATTTTAGAAGAAACAGGTAATGTCGTTCTGTCGCATCCATCAGTATTCACGATCTCATTTACTCAAGCTTCTGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAAGATGAGGTGGTACAAGCATTGATGGACGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATATCCATATTTAGCGGCCTCACAAGTCGGTACAA
AGCAGTCTGTTTCTGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGCTCGT
ACAACGTTGTATGACCGTCCGGCTTTCATGAAGAAAAAAGCACTGACTGCTGCAGAGCAAGGCACCGCAATGCACACAGT
CATGCAGCATATTCCGCTTCCTTCTGATGTGCTCTATGATGATTCACGTATACAGCAACTGCTCGATTCCTTACAGCAGA
GAGACTTACTAACAAATGAACAAGTCCAGTCAATTGATCAAGAAGGAATTACAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCAAAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGAGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAGCTATATTTACTAG
ATTATAAAACCGATAGAGTGCAAGGCAGATATACAGGCGGCCTTAAAGCAGCTGCGCCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
CGGGAATTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.019

100

0.661