Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   R9P_RS02045 Genome accession   NZ_AP024582
Coordinates   439904..440581 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain Ec387     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434904..445581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R9P_RS02030 (R9P_04030) chiA 436271..438964 (+) 2694 WP_001402584.1 bifunctional chitinase/lysozyme -
  R9P_RS02035 (R9P_04040) bfd 439133..439327 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  R9P_RS02040 (R9P_04050) bfr 439399..439875 (+) 477 WP_000675504.1 bacterioferritin -
  R9P_RS02045 (R9P_04060) pilD 439904..440581 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  R9P_RS02050 (R9P_04070) gspM 440581..441042 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  R9P_RS02055 (R9P_04080) gspL 441039..442202 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  R9P_RS02060 (R9P_04090) gspK 442217..443200 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  R9P_RS02065 (R9P_04100) gspJ 443193..443780 (-) 588 WP_001402582.1 type II secretion system minor pseudopilin GspJ -
  R9P_RS02070 (R9P_04110) gspI 443773..444150 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  R9P_RS02075 (R9P_04120) gspH 444147..444656 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  R9P_RS02080 (R9P_04130) gspG 444664..445101 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=73370 R9P_RS02045 WP_000178161.1 439904..440581(-) (pilD) [Escherichia coli strain Ec387]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=73370 R9P_RS02045 WP_000178161.1 439904..440581(-) (pilD) [Escherichia coli strain Ec387]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment