Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N8O08_RS02125 Genome accession   NZ_CP104976
Coordinates   459214..460647 (-) Length   477 a.a.
NCBI ID   WP_223561679.1    Uniprot ID   -
Organism   Enterobacter sp. 155105     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454214..465647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8O08_RS02095 (N8O08_02095) elbB 455746..456399 (+) 654 WP_223563540.1 isoprenoid biosynthesis glyoxalase ElbB -
  N8O08_RS02100 (N8O08_02100) mtgA 456396..457121 (+) 726 WP_223561681.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N8O08_RS02105 (N8O08_02105) npr 457161..457433 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  N8O08_RS02110 (N8O08_02110) rapZ 457430..458284 (-) 855 WP_223561680.1 RNase adapter RapZ -
  N8O08_RS02115 (N8O08_02115) ptsN 458330..458821 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N8O08_RS02120 (N8O08_02120) hpf 458904..459191 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  N8O08_RS02125 (N8O08_02125) rpoN/rpoN1 459214..460647 (-) 1434 WP_223561679.1 RNA polymerase factor sigma-54 Machinery gene
  N8O08_RS02130 (N8O08_02130) lptB 460695..461420 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  N8O08_RS02135 (N8O08_02135) lptA 461427..461981 (-) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N8O08_RS02140 (N8O08_02140) lptC 461950..462525 (-) 576 WP_223561678.1 LPS export ABC transporter periplasmic protein LptC -
  N8O08_RS02145 (N8O08_02145) kdsC 462522..463088 (-) 567 WP_223563539.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N8O08_RS02150 (N8O08_02150) kdsD 463103..464089 (-) 987 WP_223561677.1 arabinose-5-phosphate isomerase KdsD -
  N8O08_RS02155 (N8O08_02155) - 464103..465080 (-) 978 WP_223561676.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54183.92 Da        Isoelectric Point: 4.3149

>NTDB_id=733636 N8O08_RS02125 WP_223561679.1 459214..460647(-) (rpoN/rpoN1) [Enterobacter sp. 155105]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPIGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=733636 N8O08_RS02125 WP_223561679.1 459214..460647(-) (rpoN/rpoN1) [Enterobacter sp. 155105]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCTCAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCAAGCTGGGATGAAATTTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACTCCCTTCT
CCGATACCGACCGCGCGATTGCCACGTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTTGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GATAGGCGTGGCCGCAAAAGATCTGCGTGACTGCCTGCTGATCCAGCTTTCTCAATTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAATACGTCATTCCGGACGTACTGGTCAGAAAACATAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAATCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCTTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAAATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGACGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48