Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5661_RS13610 Genome accession   NZ_CP120600
Coordinates   2568433..2568843 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain PRO112     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2563433..2573843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5661_RS13580 (P5661_13580) - 2563519..2563659 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  P5661_RS13585 (P5661_13585) yqeF 2563965..2564696 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  P5661_RS13590 (P5661_13590) cwlH 2564948..2565700 (-) 753 WP_277708804.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  P5661_RS13595 (P5661_13595) yqeD 2565887..2566513 (+) 627 WP_161619430.1 TVP38/TMEM64 family protein -
  P5661_RS13600 (P5661_13600) gnd 2566533..2567426 (-) 894 WP_038829699.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  P5661_RS13605 (P5661_13605) yqeB 2567678..2568400 (+) 723 WP_277708805.1 DUF308 domain-containing protein -
  P5661_RS13610 (P5661_13610) nucA/comI 2568433..2568843 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  P5661_RS13615 (P5661_13615) - 2569039..2569389 (+) 351 Protein_2630 sigma-70 family RNA polymerase sigma factor -
  P5661_RS13620 (P5661_13620) spoIVCA 2569416..2570876 (-) 1461 WP_250620103.1 site-specific DNA recombinase SpoIVCA -
  P5661_RS13625 (P5661_13625) - 2570834..2571012 (-) 179 Protein_2632 hypothetical protein -
  P5661_RS13630 (P5661_13630) - 2571451..2572386 (-) 936 WP_277708806.1 DMT family transporter -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=733002 P5661_RS13610 WP_009967785.1 2568433..2568843(-) (nucA/comI) [Bacillus subtilis strain PRO112]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=733002 P5661_RS13610 WP_009967785.1 2568433..2568843(-) (nucA/comI) [Bacillus subtilis strain PRO112]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529