Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PALA6_RS26395 Genome accession   NZ_CP104867
Coordinates   5671682..5673814 (-) Length   710 a.a.
NCBI ID   WP_269975356.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5666682..5678814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA6_RS26380 (PALA6_05265) - 5668334..5669989 (-) 1656 WP_004347588.1 AAA family ATPase -
  PALA6_RS26385 (PALA6_05266) aroB 5669996..5671102 (-) 1107 WP_004347587.1 3-dehydroquinate synthase -
  PALA6_RS26390 (PALA6_05267) aroK 5671152..5671670 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PALA6_RS26395 (PALA6_05268) pilQ 5671682..5673814 (-) 2133 WP_269975356.1 type 4a pilus secretin PilQ Machinery gene
  PALA6_RS26400 (PALA6_05269) pilP 5673868..5674392 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  PALA6_RS26405 (PALA6_05270) pilO 5674389..5675012 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  PALA6_RS26410 (PALA6_05271) pilN 5675009..5675605 (-) 597 WP_269975357.1 type 4a pilus biogenesis protein PilN -
  PALA6_RS26415 (PALA6_05272) pilM 5675605..5676669 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 710 a.a.        Molecular weight: 77334.86 Da        Isoelectric Point: 5.3155

>NTDB_id=732940 PALA6_RS26395 WP_269975356.1 5671682..5673814(-) (pilQ) [Pseudomonas aeruginosa strain PALA6]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTMEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGSVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SSGEEKENSRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHNGNWNS
YGKNGNMGIKDKEGYNCGPFQGQCTFPTRDNSPTPFVDMGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEVVSQPKV
VTSDKETAKILKGSEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFQNALNGVPPINKNEVNA
KILVNDGETIVIGGVFSNTQTKAVDKVPFLGDLPYLGRLFRRDTVSDTKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2133 bp        

>NTDB_id=732940 PALA6_RS26395 WP_269975356.1 5671682..5673814(-) (pilQ) [Pseudomonas aeruginosa strain PALA6]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCGTTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGAGACCGGGTCGAGCTAAAGTTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
TTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACGCGGTTGATCATCAACCTGACCACGCT
GTCGTCCTACACTACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCTGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATTCAAGAACAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAACCGATGACCATGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCTGATACCGTGCAGGGCAATATCACCCTG
CGTCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAG
CGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATTGCCAAGCTGTTCCAGTCGGTTACT
AGTAGTGGCGAAGAGAAAGAAAATTCTCGTGGTTCGATTACCGTAGATGACCGTACCAACAGCATCATTGCCTATCAGCC
GCAGGAGCGGCTGGACGAGCTACGCCGAATCGTTTCCCAGTTGGACATCCCGGTTCGTCAGGTGATGATCGAGGCCCGTA
TCGTCGAGGCCAACGTGGGGTACGACAAGAGTCTTGGTGTGCGTTGGGGTGGTGCCTACCACAATGGCAACTGGAACAGC
TACGGCAAGAACGGCAACATGGGCATCAAGGATAAGGAAGGTTACAACTGCGGGCCGTTCCAGGGGCAGTGTACGTTCCC
GACCAGAGATAATTCTCCGACCCCATTTGTGGATATGGGGGCCAAGGATGCTACTTCCGGTATCGGTATTGGCTTTATCA
CCGATAACATCATTCTTGACTTGCAATTATCGGCCATGGAGAAAACCGGGAACGGCGAAGTGGTTTCGCAACCCAAGGTC
GTAACTTCTGACAAGGAAACCGCGAAGATCCTAAAGGGATCGGAGATCCCATATCAGGAGGCCAGTTCCAGTGGTGCGAC
CAGCACCTCGTTCAAGGAGGCCGCTCTTTCTCTGGAGGTGACGCCGCAGATCACTCCAGACAATCGGATCATCGTTGAGG
TGAAGGTGACCAAGGATGCTCCCGACTTCCAGAATGCTTTGAATGGAGTTCCTCCGATCAACAAGAACGAGGTTAACGCC
AAGATTCTGGTCAACGATGGTGAAACCATCGTTATCGGCGGTGTATTCAGCAACACCCAGACAAAGGCTGTCGACAAGGT
ACCCTTCCTCGGTGACCTGCCTTACTTGGGCCGTTTGTTCCGCCGCGATACGGTCTCCGACACGAAAAACGAACTTCTGG
TTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(545-701)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

94.678

100

0.952
  comQ Acinetobacter baylyi ADP1

42.225

98.732

0.417
  pilQ Acinetobacter baumannii strain A118

40.48

99.859

0.404
  pilQ Acinetobacter baumannii D1279779

40.48

99.859

0.404
  pilQ Legionella pneumophila strain Lp02

37.956

96.479

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.956

96.479

0.366