Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5622_RS11715 Genome accession   NZ_CP120598
Coordinates   2221385..2221795 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain PRO115     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2216385..2226795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5622_RS11680 (P5622_11680) arsR 2216549..2216866 (+) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -
  P5622_RS11685 (P5622_11685) yqcK 2216927..2217367 (+) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  P5622_RS11690 (P5622_11690) acr3 2217390..2218430 (+) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  P5622_RS11695 (P5622_11695) arsC 2218442..2218861 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  P5622_RS11700 (P5622_11700) - 2219216..2219394 (+) 179 Protein_2247 hypothetical protein -
  P5622_RS11705 (P5622_11705) spoIVCA 2219352..2220812 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  P5622_RS11710 (P5622_11710) - 2220839..2221189 (-) 351 Protein_2249 sigma-70 family RNA polymerase sigma factor -
  P5622_RS11715 (P5622_11715) nucA/comI 2221385..2221795 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  P5622_RS11720 (P5622_11720) yqeB 2221828..2222550 (-) 723 WP_010886572.1 hypothetical protein -
  P5622_RS11725 (P5622_11725) gnd 2222802..2223740 (+) 939 WP_063694977.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  P5622_RS11730 (P5622_11730) yqeD 2223713..2224338 (-) 626 Protein_2253 TVP38/TMEM64 family protein -
  P5622_RS11735 (P5622_11735) cwlH 2224525..2225277 (+) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  P5622_RS11740 (P5622_11740) yqeF 2225529..2226260 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  P5622_RS11745 (P5622_11745) - 2226566..2226706 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=732835 P5622_RS11715 WP_009967785.1 2221385..2221795(+) (nucA/comI) [Bacillus subtilis strain PRO115]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=732835 P5622_RS11715 WP_009967785.1 2221385..2221795(+) (nucA/comI) [Bacillus subtilis strain PRO115]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529