Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5628_RS12905 Genome accession   NZ_CP120577
Coordinates   2479210..2479620 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain PRO53     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2474210..2484620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5628_RS12875 (P5628_12875) - 2474296..2474436 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  P5628_RS12880 (P5628_12880) yqeF 2474742..2475473 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  P5628_RS12885 (P5628_12885) cwlH 2475725..2476477 (-) 753 WP_277708804.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  P5628_RS12890 (P5628_12890) yqeD 2476664..2477290 (+) 627 WP_161619430.1 TVP38/TMEM64 family protein -
  P5628_RS12895 (P5628_12895) gnd 2477310..2478203 (-) 894 WP_038829699.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  P5628_RS12900 (P5628_12900) yqeB 2478455..2479177 (+) 723 WP_277708805.1 DUF308 domain-containing protein -
  P5628_RS12905 (P5628_12905) nucA/comI 2479210..2479620 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  P5628_RS12910 (P5628_12910) - 2479816..2480163 (+) 348 Protein_2498 sigma-70 family RNA polymerase sigma factor -
  P5628_RS12915 (P5628_12915) spoIVCA 2480194..2481654 (-) 1461 WP_250620103.1 site-specific DNA recombinase SpoIVCA -
  P5628_RS12920 (P5628_12920) - 2481612..2481790 (-) 179 Protein_2500 hypothetical protein -
  P5628_RS12925 (P5628_12925) - 2482229..2483164 (-) 936 WP_277708806.1 DMT family transporter -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=732714 P5628_RS12905 WP_009967785.1 2479210..2479620(-) (nucA/comI) [Bacillus subtilis strain PRO53]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=732714 P5628_RS12905 WP_009967785.1 2479210..2479620(-) (nucA/comI) [Bacillus subtilis strain PRO53]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529