Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FLY91_RS06895 Genome accession   NZ_AP024560
Coordinates   1465528..1466205 (-) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain NIPH17_0020     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1460528..1471205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLY91_RS06880 chiA 1461895..1464588 (+) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -
  FLY91_RS06885 bfd 1464757..1464951 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  FLY91_RS06890 bfr 1465023..1465499 (+) 477 WP_000675504.1 bacterioferritin -
  FLY91_RS06895 pilD 1465528..1466205 (-) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  FLY91_RS06900 gspM 1466205..1466666 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  FLY91_RS06905 gspL 1466663..1467826 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  FLY91_RS06910 gspK 1467841..1468824 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  FLY91_RS06915 gspJ 1468817..1469404 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  FLY91_RS06920 gspI 1469397..1469774 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  FLY91_RS06925 gspH 1469771..1470280 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  FLY91_RS06930 gspG 1470288..1470725 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=73235 FLY91_RS06895 WP_001377644.1 1465528..1466205(-) (pilD) [Escherichia coli strain NIPH17_0020]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=73235 FLY91_RS06895 WP_001377644.1 1465528..1466205(-) (pilD) [Escherichia coli strain NIPH17_0020]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373

Multiple sequence alignment