Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   N5P16_RS16245 Genome accession   NZ_CP104734
Coordinates   3482673..3483473 (-) Length   266 a.a.
NCBI ID   WP_011182074.1    Uniprot ID   A0A7G2SCF1
Organism   Acinetobacter baylyi strain JAT2044     
Function   assists with secretin assembly and localization (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3477673..3488473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5P16_RS16225 (N5P16_16225) - 3478739..3479440 (-) 702 WP_004919988.1 YfgM family protein -
  N5P16_RS16230 (N5P16_16230) hisS 3479472..3480764 (-) 1293 WP_004919990.1 histidine--tRNA ligase -
  N5P16_RS16235 (N5P16_16235) ispG 3480761..3481876 (-) 1116 WP_004919993.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  N5P16_RS16240 (N5P16_16240) - 3481900..3482682 (-) 783 WP_004919996.1 helix-turn-helix domain-containing protein -
  N5P16_RS16245 (N5P16_16245) pilF 3482673..3483473 (-) 801 WP_011182074.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  N5P16_RS16250 (N5P16_16250) rlmN 3483492..3484736 (-) 1245 WP_004920007.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  N5P16_RS16255 (N5P16_16255) ndk 3484859..3485290 (-) 432 WP_004920009.1 nucleoside-diphosphate kinase -
  N5P16_RS16260 (N5P16_16260) iscX 3485401..3485601 (-) 201 WP_004920012.1 Fe-S cluster assembly protein IscX -
  N5P16_RS16265 (N5P16_16265) - 3485673..3486224 (-) 552 WP_026056982.1 phosphatase PAP2 family protein -
  N5P16_RS16270 (N5P16_16270) - 3486259..3487548 (-) 1290 WP_011182072.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29877.99 Da        Isoelectric Point: 9.5059

>NTDB_id=731713 N5P16_RS16245 WP_011182074.1 3482673..3483473(-) (pilF) [Acinetobacter baylyi strain JAT2044]
MSKWTINKTLGCAAIVGAFILSGCQTVPTTKDPEKGVKVRTQLAAEYIKSGDLDSAKRALDQALKVNPKDSSANMMMGVL
LQQEGSRPNLEKADAYFERAVQLDPENAQAHNNYGTYLYQMKRYHDAIQQLTLAGSSLGYDQRYRALENIGRIYLHLGGV
ANAEKMFSQALQANRDSSVSMLELAEIYYLQQRIPAATGMYEQYVRTVGQKNQGARALWIGIRVARANADRTGVQVLVNQ
LRIFYPDSSEYQRYLKLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=731713 N5P16_RS16245 WP_011182074.1 3482673..3483473(-) (pilF) [Acinetobacter baylyi strain JAT2044]
TTGAGCAAGTGGACAATAAACAAAACGTTGGGTTGTGCCGCCATTGTAGGGGCGTTTATTTTGTCTGGATGTCAGACTGT
TCCAACCACCAAAGACCCTGAAAAGGGTGTAAAAGTACGTACTCAACTTGCTGCCGAATATATTAAATCTGGTGATCTTG
ATTCGGCTAAACGTGCTTTAGATCAAGCCTTAAAAGTAAATCCCAAAGATAGCAGTGCCAACATGATGATGGGTGTGCTG
CTGCAACAAGAAGGTAGTCGCCCTAACCTTGAAAAAGCAGATGCTTATTTTGAGCGAGCTGTACAGCTTGATCCAGAAAA
TGCACAAGCGCATAACAACTATGGTACCTATTTGTATCAGATGAAACGTTATCATGATGCAATTCAACAATTGACACTGG
CTGGTAGTTCATTAGGATATGACCAGCGCTATCGTGCATTGGAAAATATTGGGCGAATTTATTTGCATCTGGGTGGAGTC
GCAAATGCTGAAAAAATGTTCAGTCAGGCGCTTCAGGCAAATCGTGATTCTTCCGTTTCGATGTTAGAGTTAGCAGAGAT
TTATTATTTGCAACAAAGAATTCCTGCCGCAACAGGTATGTACGAACAATATGTGCGTACAGTTGGGCAGAAAAATCAGG
GTGCGCGAGCACTCTGGATTGGTATTCGTGTGGCGAGAGCCAATGCCGATCGGACTGGGGTACAGGTGTTAGTTAATCAG
CTTCGTATTTTTTACCCAGATAGTTCAGAATATCAACGTTATTTGAAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-70)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_16)

(150-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2SCF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baylyi ADP1

100

100

1
  pilF Acinetobacter baumannii D1279779

74.231

97.744

0.726