Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N5934_RS13860 Genome accession   NZ_CP104691
Coordinates   2837739..2839172 (-) Length   477 a.a.
NCBI ID   WP_023295498.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 2022CK-00453     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2832739..2844172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5934_RS13830 (N5934_13830) elbB 2834271..2834924 (+) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -
  N5934_RS13835 (N5934_13835) mtgA 2834921..2835646 (+) 726 WP_023295499.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N5934_RS13840 (N5934_13840) npr 2835686..2835958 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  N5934_RS13845 (N5934_13845) rapZ 2835955..2836809 (-) 855 WP_003861870.1 RNase adapter RapZ -
  N5934_RS13850 (N5934_13850) ptsN 2836855..2837346 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N5934_RS13855 (N5934_13855) hpf 2837429..2837716 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  N5934_RS13860 (N5934_13860) rpoN/rpoN1 2837739..2839172 (-) 1434 WP_023295498.1 RNA polymerase factor sigma-54 Machinery gene
  N5934_RS13865 (N5934_13865) lptB 2839220..2839945 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  N5934_RS13870 (N5934_13870) lptA 2839952..2840506 (-) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N5934_RS13875 (N5934_13875) lptC 2840475..2841050 (-) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  N5934_RS13880 (N5934_13880) kdsC 2841047..2841613 (-) 567 WP_110871438.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N5934_RS13885 (N5934_13885) kdsD 2841628..2842614 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  N5934_RS13890 (N5934_13890) - 2842628..2843605 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54098.98 Da        Isoelectric Point: 4.3549

>NTDB_id=731433 N5934_RS13860 WP_023295498.1 2837739..2839172(-) (rpoN/rpoN1) [Enterobacter hormaechei strain 2022CK-00453]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGNDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=731433 N5934_RS13860 WP_023295498.1 2837739..2839172(-) (rpoN/rpoN1) [Enterobacter hormaechei strain 2022CK-00453]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACTCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCAATGACGAGATTGAACTGGAAGAGATTGAAGCCGTCCTGAAACGCGTTCAGCGCTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAACTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCATGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
TGAACCGGAATATGTCATCCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAATGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAGGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48