Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   N5980_RS12775 Genome accession   NZ_CP104651
Coordinates   2627149..2627949 (-) Length   266 a.a.
NCBI ID   WP_175966667.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain 2021CK-01005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2622149..2632949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5980_RS12755 (N5980_12745) - 2623200..2623898 (-) 699 WP_261635020.1 YfgM family protein -
  N5980_RS12760 (N5980_12750) hisS 2623929..2625224 (-) 1296 WP_005232127.1 histidine--tRNA ligase -
  N5980_RS12765 (N5980_12755) ispG 2625228..2626349 (-) 1122 WP_005232130.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  N5980_RS12770 (N5980_12760) - 2626373..2627158 (-) 786 WP_005232131.1 helix-turn-helix domain-containing protein -
  N5980_RS12775 (N5980_12765) pilF 2627149..2627949 (-) 801 WP_175966667.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  N5980_RS12780 (N5980_12770) rlmN 2627964..2629199 (-) 1236 WP_005232136.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  N5980_RS12785 (N5980_12775) ndk 2629321..2629752 (-) 432 WP_004782097.1 nucleoside-diphosphate kinase -
  N5980_RS12790 (N5980_12780) iscX 2629862..2630062 (-) 201 WP_005232138.1 Fe-S cluster assembly protein IscX -
  N5980_RS12795 (N5980_12785) - 2630151..2630702 (-) 552 WP_004782094.1 phosphatase PAP2 family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29962.06 Da        Isoelectric Point: 9.0592

>NTDB_id=731259 N5980_RS12775 WP_175966667.1 2627149..2627949(-) (pilF) [Acinetobacter variabilis strain 2021CK-01005]
MSNLTSKLALVSVVCGSLVLTACQTPDLGQKDPEKATKVRTQLAVEYLKNGDLDSAKRALDQALETNPRDSQANMMMGVL
LQQEGSKPNLEKAEGYFKRAISADPKNAQARNNYGTYLYQLERYKDAIEQFEIAGATLGYDQRFRALENMGRIYLKQGDL
VNAEKSFKQALQANRASYISMLELAEIFYLNQQFPAATQMYEQFVRGAGQKNQSARALWIGIRTARASGDQLGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=731259 N5980_RS12775 WP_175966667.1 2627149..2627949(-) (pilF) [Acinetobacter variabilis strain 2021CK-01005]
TTGAGTAATCTGACATCTAAACTTGCTTTGGTTTCAGTAGTATGTGGGTCTTTGGTTTTAACTGCGTGTCAAACGCCAGA
TCTGGGTCAGAAGGACCCGGAAAAAGCCACCAAGGTTCGTACTCAGTTGGCGGTTGAATATCTTAAAAACGGTGATCTGG
ATTCAGCTAAACGTGCGCTGGATCAGGCACTGGAAACTAATCCGCGTGATTCGCAAGCCAATATGATGATGGGCGTGCTG
CTTCAGCAGGAAGGCAGCAAACCTAATCTTGAAAAGGCCGAAGGTTATTTCAAGCGTGCAATTTCGGCAGATCCCAAAAA
TGCCCAGGCACGAAATAACTACGGCACCTATTTATATCAGCTTGAGCGCTATAAAGATGCGATCGAGCAGTTCGAAATTG
CCGGCGCAACTTTAGGTTATGACCAGCGATTTCGTGCCCTTGAAAATATGGGACGGATTTACCTGAAACAGGGTGATTTG
GTGAATGCTGAAAAATCATTCAAACAGGCACTACAGGCTAATCGCGCTTCGTATATATCAATGTTAGAGTTGGCAGAAAT
TTTTTATCTGAACCAGCAGTTCCCGGCCGCGACTCAAATGTATGAACAGTTTGTACGTGGCGCGGGGCAGAAAAACCAGA
GTGCCCGTGCGCTCTGGATTGGCATTCGCACTGCGCGTGCCAGTGGCGATCAGTTAGGCATGCAGGTATTGGTCAATCAA
TTACGTGCATTGTTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(145-174)

Tetratricopeptide repeat (TPR_14)

(40-79)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.316

100

0.763
  pilF Acinetobacter baylyi ADP1

69.288

100

0.695