Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5930_RS05380 Genome accession   NZ_CP104643
Coordinates   1122744..1123724 (+) Length   326 a.a.
NCBI ID   WP_001055642.1    Uniprot ID   A0A3V9WZJ6
Organism   Salmonella enterica strain 1020677     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1117744..1128724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5930_RS05350 (N5930_05355) - 1118322..1119329 (+) 1008 WP_000252203.1 DUF1202 domain-containing protein -
  N5930_RS05355 (N5930_05360) hemW 1119420..1120556 (-) 1137 WP_001096521.1 radical SAM family heme chaperone HemW -
  N5930_RS05360 (N5930_05365) - 1120549..1121142 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  N5930_RS05365 (N5930_05370) yggU 1121150..1121440 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  N5930_RS05370 (N5930_05375) - 1121437..1122003 (-) 567 WP_001094848.1 YggT family protein -
  N5930_RS05375 (N5930_05380) - 1122022..1122726 (-) 705 WP_000651540.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5930_RS05380 (N5930_05385) pilT 1122744..1123724 (+) 981 WP_001055642.1 type IV pilus twitching motility protein PilT Machinery gene
  N5930_RS05385 (N5930_05390) - 1123856..1124542 (+) 687 WP_000098325.1 IclR family transcriptional regulator -
  N5930_RS05390 (N5930_05395) ruvX 1124589..1125005 (-) 417 WP_001285490.1 Holliday junction resolvase RuvX -
  N5930_RS05395 (N5930_05400) - 1125005..1125568 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  N5930_RS05400 (N5930_05405) gshB 1125784..1126731 (-) 948 WP_000593248.1 glutathione synthase -
  N5930_RS05405 (N5930_05410) rsmE 1126751..1127482 (-) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N5930_RS05410 (N5930_05415) endA 1127559..1128266 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36125.28 Da        Isoelectric Point: 7.5355

>NTDB_id=731141 N5930_RS05380 WP_001055642.1 1122744..1123724(+) (pilT) [Salmonella enterica strain 1020677]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=731141 N5930_RS05380 WP_001055642.1 1122744..1123724(+) (pilT) [Salmonella enterica strain 1020677]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3V9WZJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365