Detailed information NTG0731038

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N5857_RS20770
• Genome accession: NZ_CP104627
• Coordinates: 4262549..4263349 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain AHM21C2836KI
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4257549..4268349

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5857_RS20735 (N5857_20730)	gspH	4257705..4258220 (+)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-
N5857_RS20740 (N5857_20735)	gspI	4258217..4258582 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
N5857_RS20745 (N5857_20740)	gspJ	4258579..4259175 (+)	597	WP_004178612.1	type II secretion system minor pseudopilin GspJ	-
N5857_RS20750 (N5857_20745)	gspK	4259172..4260158 (+)	987	WP_004213439.1	type II secretion system minor pseudopilin GspK	-
N5857_RS20755 (N5857_20750)	gspL	4260145..4261338 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
N5857_RS20760 (N5857_20755)	-	4261331..4261816 (+)	486	WP_004145896.1	type II secretion system protein M	-
N5857_RS20765 (N5857_20760)	-	4261806..4262552 (+)	747	WP_023316460.1	type II secretion system protein N	-
N5857_RS20770 (N5857_20765)	pilD	4262549..4263349 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
N5857_RS20775 (N5857_20770)	hrpB	4263397..4265826 (-)	2430	WP_135729565.1	ATP-dependent helicase HrpB	-
N5857_RS20780 (N5857_20775)	thpR	4265899..4266435 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
N5857_RS20785 (N5857_20780)	sfsA	4266435..4267151 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
N5857_RS20790 (N5857_20785)	dksA	4267314..4267769 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=731038 N5857_RS20770 WP_004145898.1 4262549..4263349(+) (pilD) [Klebsiella pneumoniae strain AHM21C2836KI]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=731038 N5857_RS20770 WP_004145898.1 4262549..4263349(+) (pilD) [Klebsiella pneumoniae strain AHM21C2836KI]
ATGACGACACTAGCTGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425