Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N5O88_RS19525 Genome accession   NZ_CP104583
Coordinates   4060066..4062180 (-) Length   704 a.a.
NCBI ID   WP_279533202.1    Uniprot ID   -
Organism   Pseudomonas sp. GD03721     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4055066..4067180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O88_RS19510 (N5O88_19500) - 4056769..4058376 (-) 1608 WP_279531404.1 SPOR domain-containing protein -
  N5O88_RS19515 (N5O88_19505) aroB 4058387..4059490 (-) 1104 WP_279531405.1 3-dehydroquinate synthase -
  N5O88_RS19520 (N5O88_19510) aroK 4059542..4060060 (-) 519 WP_084341405.1 shikimate kinase AroK -
  N5O88_RS19525 (N5O88_19515) pilQ 4060066..4062180 (-) 2115 WP_279533202.1 type IV pilus secretin PilQ Machinery gene
  N5O88_RS19530 (N5O88_19520) pilP 4062236..4062760 (-) 525 WP_279531406.1 type 4a pilus biogenesis lipoprotein PilP -
  N5O88_RS19535 (N5O88_19525) pilO 4062760..4063380 (-) 621 WP_279531407.1 type 4a pilus biogenesis protein PilO -
  N5O88_RS19540 (N5O88_19530) - 4063377..4063949 (-) 573 WP_279531408.1 PilN domain-containing protein -
  N5O88_RS19545 (N5O88_19535) pilM 4063949..4065013 (-) 1065 WP_037004588.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 76099.51 Da        Isoelectric Point: 4.7719

>NTDB_id=730748 N5O88_RS19525 WP_279533202.1 4060066..4062180(-) (pilQ) [Pseudomonas sp. GD03721]
MNGSLSRLSVALMAALLSPAVLAANLQGLDVASLPGDRVELKLSFDEPVTAPRGYTIEQPARIALDLPGVSNKLGSKNRE
LGVGNARSVTIVEAKDRTRLIVNLTKLAPYSTRVEGNDLFVLVGDTSSLTSRPAAVSAVTNTVPAKKVYGPQPKAINNID
FQRGEQGEGNVIITLSDKSVAPDIQEESGKIRLDFVKTELPEFLRVRLDVKDFATPVQFVSATGSADKTSIVIEPVGLYD
YLAYQTDNTLTLSVKPLSQEDAEQRKAERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAAEMAKLFQSVTSADGASD
VRGSVTVDERTNSIIAYQTQDRLDELRRIIAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGATRRGNWSVYGKDGATS
FDSDSGQVFLPGTDTVGSVTLQDGVAPVPFVDMGVLGSTSGIGIGFLTDNLTLDLQLSAMEKTGNGEIVSQPKVVTSDKE
TAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFARALNGVPPINKNEVNAKVLVND
GETIVIGGVFSNTQTKGVEKVPLLGDLPFLGRVFRRDIVSDNKAELLVFITPRIMNNQAIAVNR

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=730748 N5O88_RS19525 WP_279533202.1 4060066..4062180(-) (pilQ) [Pseudomonas sp. GD03721]
ATGAATGGCTCTCTTTCGCGTCTTAGCGTCGCCCTGATGGCTGCGCTGCTGTCACCCGCAGTCTTGGCGGCTAACTTACA
GGGTCTGGATGTTGCCAGTTTGCCCGGTGATAGGGTTGAGTTGAAACTGTCCTTCGATGAGCCGGTAACGGCTCCTCGCG
GCTATACCATCGAGCAGCCTGCGCGTATTGCTCTGGACCTTCCGGGTGTGTCGAACAAACTGGGCTCCAAGAACAGAGAG
CTGGGCGTCGGAAATGCGCGCAGTGTGACCATCGTCGAAGCCAAGGACCGTACCCGGCTTATTGTCAATCTGACCAAGCT
CGCGCCTTACAGCACGCGTGTCGAAGGTAACGATCTGTTCGTTCTGGTCGGTGACACTTCCTCTTTAACATCGCGTCCTG
CTGCAGTATCTGCTGTAACCAATACAGTGCCTGCCAAGAAAGTCTACGGTCCGCAGCCCAAGGCAATCAACAATATTGAT
TTTCAGCGTGGTGAACAGGGTGAAGGCAATGTCATTATCACGCTTTCTGATAAGTCAGTTGCGCCTGACATCCAAGAGGA
GAGTGGTAAGATTCGTCTTGATTTTGTGAAGACGGAATTGCCTGAATTTCTGAGAGTTCGTCTTGATGTCAAGGACTTTG
CGACTCCGGTGCAGTTCGTTAGTGCCACGGGTAGCGCAGACAAAACCAGTATCGTGATCGAACCAGTTGGTTTATACGAT
TACCTGGCTTACCAGACTGACAACACGCTGACCTTGAGTGTTAAGCCTCTTTCCCAGGAGGACGCCGAACAGCGTAAAGC
TGAGCGATTTGCTTATACCGGGGAGAAACTGTCGCTTAACTTCCAGGATATCGACGTGCGCTCGGTCTTGCAGTTGATCG
CTGACTTCACTGACTTGAATCTGGTGGCAAGTGACACTGTTGCGGGTAATATCACTTTACGTCTGCAGAATGTGCCATGG
GATCAGGCGTTGGATTTGGTTTTGAAGACCAAGGGCTTGGATAAGCGTCAAGTTGGTAACGTCTTGCTTGTTGCCCCGGC
TGATGAAATCGCAGCGCGTGAGCGCCAGGAATTGGAATCGCAAAAGCAAATTGCTGAGCTGGCTCCGCTGCGGCGTGAAC
TGATTCAGGTAAATTATGCCAAGGCCGCTGAAATGGCCAAACTGTTTCAGTCTGTTACCAGTGCCGACGGTGCTTCAGAT
GTTCGTGGGTCTGTAACGGTCGATGAACGAACCAACAGCATCATTGCTTACCAGACGCAGGATCGCCTGGATGAGTTGAG
GCGAATCATCGCCCAGCTCGATATCCCTGTGCGTCAGGTAATGATCGAGGCGCGCATTGTTGAGGCTAATGTGGATTATG
ACAAGGCCTTGGGGGTGCGTTGGGGGGGGGCAACTCGTCGCGGAAACTGGAGCGTTTACGGCAAGGATGGTGCCACCTCT
TTCGATAGTGATAGTGGGCAGGTCTTTTTGCCGGGTACCGATACGGTAGGCAGCGTTACGCTGCAGGATGGCGTGGCTCC
TGTGCCCTTCGTCGATATGGGTGTTCTTGGTAGTACTTCGGGTATCGGTATCGGTTTCCTTACTGATAATTTGACGCTTG
ACCTGCAGCTGTCGGCTATGGAAAAGACGGGCAATGGCGAGATTGTTTCTCAACCCAAGGTTGTGACGTCGGATAAGGAA
ACTGCGAAGATCCTGAAGGGGCAGGAAGTACCCTATCAGGAGGCCAGTTCTAGTGGTGCCACCAGTACATCCTTCAAGGA
GGCAGCGCTCTCACTGGAGGTTACGCCGCAGATTACCCCGGATAATCGCATCATCATGGAGGTGAAAGTAACCAAGGATG
CGCCTGACTTTGCTCGGGCTCTTAATGGTGTGCCGCCAATCAACAAGAACGAAGTCAATGCCAAGGTACTGGTCAACGAC
GGTGAGACCATTGTGATTGGTGGGGTATTCTCCAATACCCAGACTAAGGGTGTAGAGAAAGTGCCACTTCTCGGTGATCT
GCCGTTCCTTGGGCGTGTATTCCGTCGTGATATCGTCAGTGACAACAAAGCCGAGTTGCTGGTTTTCATCACTCCGCGCA
TCATGAACAACCAAGCCATTGCCGTGAACCGATGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(539-695)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.698

100

0.77
  comQ Acinetobacter baylyi ADP1

41.429

99.432

0.412
  pilQ Acinetobacter baumannii strain A118

39.291

100

0.409
  pilQ Acinetobacter baumannii D1279779

39.291

100

0.409
  pilQ Legionella pneumophila strain ERS1305867

38.405

96.165

0.369
  pilQ Legionella pneumophila strain Lp02

38.257

96.165

0.368