Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5O83_RS20185 Genome accession   NZ_CP104579
Coordinates   4141132..4142448 (-) Length   438 a.a.
NCBI ID   WP_003463985.1    Uniprot ID   W6R805
Organism   Pseudomonas oleovorans strain GD04132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4136132..4147448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O83_RS20150 (N5O83_20145) thiS 4136215..4136415 (-) 201 WP_045735689.1 sulfur carrier protein ThiS -
  N5O83_RS20155 (N5O83_20150) - 4136518..4136889 (-) 372 WP_003463966.1 DUF423 domain-containing protein -
  N5O83_RS20160 (N5O83_20155) mtgA 4137050..4137777 (+) 728 Protein_3958 monofunctional biosynthetic peptidoglycan transglycosylase -
  N5O83_RS20165 (N5O83_20160) - 4137779..4138390 (-) 612 WP_128578750.1 hypothetical protein -
  N5O83_RS20170 (N5O83_20165) rpoH 4138453..4139307 (-) 855 WP_084340426.1 RNA polymerase sigma factor RpoH -
  N5O83_RS20175 (N5O83_20170) ftsX 4139451..4140467 (-) 1017 WP_206408652.1 permease-like cell division protein FtsX -
  N5O83_RS20180 (N5O83_20175) ftsE 4140467..4141135 (-) 669 WP_003463983.1 cell division ATP-binding protein FtsE -
  N5O83_RS20185 (N5O83_20180) pilA 4141132..4142448 (-) 1317 WP_003463985.1 signal recognition particle-docking protein FtsY Machinery gene
  N5O83_RS20190 (N5O83_20185) - 4142787..4143221 (+) 435 WP_279533921.1 BLUF domain-containing protein -
  N5O83_RS20195 (N5O83_20190) - 4143366..4144733 (+) 1368 WP_279533922.1 pitrilysin family protein -
  N5O83_RS20200 (N5O83_20195) - 4144730..4146190 (+) 1461 WP_279533923.1 pitrilysin family protein -
  N5O83_RS20205 (N5O83_20200) rsmD 4146190..4146807 (+) 618 WP_279533924.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -

Sequence

Protein


Download         Length: 438 a.a.        Molecular weight: 46144.97 Da        Isoelectric Point: 5.2212

>NTDB_id=730641 N5O83_RS20185 WP_003463985.1 4141132..4142448(-) (pilA) [Pseudomonas oleovorans strain GD04132]
MFGSKDDKNSPAVTDPQAQPVSEEKKSLFSWWRKKPAETTAEPAQPAPVEQPAAQAPVEPPVPEAVATVAVEVAEVAPAP
SASPEPHVVAPPVVAAAVQPQPVVPEPVVEAAPVAAVPAPVAAAATQEKPSFFARLKQGLSKTSASLGEGMASLFLGKKA
IDDDLLDDLETRLLTADVGVEATTAIIGNLTKRVARKELADSGALYKALQEELVTLLKPVEQPLVIDSGKQPYVILVVGV
NGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNNIAVIAQHTGADSASVIFDAVQAAKSRGIDVLIA
DTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNQTVNLTGLALTKLDGTAKGGVIFALA
KQFGLPIRYIGVGEGIDDLRPFQAQAFVQALFEERERG

Nucleotide


Download         Length: 1317 bp        

>NTDB_id=730641 N5O83_RS20185 WP_003463985.1 4141132..4142448(-) (pilA) [Pseudomonas oleovorans strain GD04132]
ATGTTTGGTTCCAAAGACGACAAGAACTCACCGGCCGTGACCGACCCCCAAGCCCAGCCCGTGAGCGAAGAAAAGAAATC
CCTGTTCAGCTGGTGGCGCAAGAAACCGGCAGAGACGACTGCCGAGCCTGCTCAGCCTGCGCCCGTTGAGCAACCTGCTG
CTCAGGCGCCTGTCGAGCCGCCCGTGCCCGAAGCCGTAGCAACGGTCGCGGTGGAAGTGGCTGAAGTCGCGCCCGCTCCG
TCAGCGTCGCCTGAGCCGCACGTCGTTGCACCGCCGGTTGTTGCAGCTGCTGTGCAGCCGCAGCCGGTCGTGCCCGAACC
TGTCGTCGAGGCAGCACCCGTGGCGGCAGTACCGGCTCCCGTCGCTGCAGCGGCCACGCAGGAAAAGCCAAGCTTCTTCG
CCCGCCTCAAGCAAGGCCTGTCCAAAACCAGCGCCAGCCTCGGTGAAGGCATGGCCAGCCTGTTCCTCGGCAAGAAGGCC
ATCGACGATGACCTGCTCGACGATCTGGAAACCCGCCTGCTCACCGCCGACGTTGGCGTCGAGGCGACCACCGCGATCAT
CGGCAACCTGACCAAGCGCGTGGCGCGCAAGGAACTGGCCGACAGCGGCGCCCTGTACAAGGCGCTGCAGGAGGAGCTGG
TGACCCTGCTCAAGCCGGTCGAGCAGCCGCTGGTCATCGACAGCGGCAAACAGCCCTATGTGATTCTCGTGGTGGGCGTG
AACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCCAAGAAACTCCAGCTCGAAGGCAAGAAGGTCATGCTCGCCGC
GGGCGACACCTTCCGCGCCGCTGCGGTGGAGCAACTGCAGGTCTGGGGCGAGCGCAACAACATTGCGGTCATCGCTCAGC
ACACCGGCGCCGATTCGGCTTCGGTGATCTTCGATGCGGTGCAGGCGGCCAAGTCGCGTGGCATCGACGTGCTTATCGCC
GACACCGCCGGGCGCCTGCACACCAAAGACAACCTGATGGAAGAGTTGAAGAAGGTCCGTCGGGTGATCGGCAAGCTGGA
CGAAACCGCGCCGCATGAGGTGCTGCTGGTACTCGATGCCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCA
ACCAGACCGTGAACCTCACCGGTCTGGCACTGACCAAACTCGACGGCACCGCCAAGGGCGGCGTTATCTTCGCCCTGGCC
AAGCAGTTCGGTCTGCCGATTCGCTATATCGGCGTGGGTGAAGGCATCGATGACCTGCGTCCGTTCCAGGCGCAGGCTTT
CGTCCAGGCACTCTTCGAGGAGCGTGAGCGCGGATGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(233-432)

SRP54-type protein, helical bundle domain (SRP54_N)

(147-213)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W6R805

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.647

97.032

0.482