Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   N5O83_RS02240 Genome accession   NZ_CP104579
Coordinates   473329..475548 (-) Length   739 a.a.
NCBI ID   WP_279534274.1    Uniprot ID   A0AA42TTZ4
Organism   Pseudomonas oleovorans strain GD04132     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468329..480548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O83_RS02220 (N5O83_02225) hslV 469021..469551 (-) 531 WP_003459006.1 ATP-dependent protease subunit HslV -
  N5O83_RS02225 (N5O83_02230) - 469771..470685 (+) 915 WP_003459008.1 NYN domain-containing protein -
  N5O83_RS02230 (N5O83_02235) - 470728..471402 (-) 675 WP_003459010.1 SPOR domain-containing protein -
  N5O83_RS02235 (N5O83_02240) argS 471403..473142 (-) 1740 WP_279534273.1 arginine--tRNA ligase -
  N5O83_RS02240 (N5O83_02245) priA 473329..475548 (-) 2220 WP_279534274.1 primosomal protein N' Machinery gene
  N5O83_RS02245 (N5O83_02250) rpmE 475707..475919 (+) 213 WP_003459015.1 50S ribosomal protein L31 -
  N5O83_RS02250 (N5O83_02255) - 475946..476746 (+) 801 WP_074855143.1 thermonuclease family protein -
  N5O83_RS02255 (N5O83_02260) - 476743..478179 (+) 1437 WP_003459017.1 M48 family metalloprotease -
  N5O83_RS02260 (N5O83_02265) - 478283..479551 (+) 1269 WP_074855142.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81996.36 Da        Isoelectric Point: 8.8782

>NTDB_id=730615 N5O83_RS02240 WP_279534274.1 473329..475548(-) (priA) [Pseudomonas oleovorans strain GD04132]
MPNLILRLALPSPLRRLFDYLAPAGVSRSALQPGARLRVPFGRREMIGVLVEVDSQSEVPLDKLKPALELLDARPPLPAS
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAETRQERYWLANKGASVDDPRLARAPRQRDALKALAQHPHGVAHSLL
SQLQLNRDSLQLLHEKGLVRVEVRRTQPHAKPAYWLAQPELPLNAEQRAAVNAVASGWSQFNAFLLAGVTGSGKTEVYLQ
LIHQCLEAGKQALVLIPEINLGPQTFDRFARRFNARIALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIVDEEHDASYKQQEGLRYHARDLALVRARQEDVPIVLGSATPSLESLHNAYTGRYALLKLTQRAGGASQPRFLRLDVKS
RPLDSGISGPMQQAIAQTLAAGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARMTVHQRYQELRCHHCGHVERQPSNC
PKCQHLDLRPVGAGTERAEERLAVLFPDYPVLRVDRDSTSRKGAMDRLFATINKGKPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSADFRASERMAQLIVQVAGRAGRAEEPGKVIIQSHLADHPLLVQLTEQGYFAFAEQALSERRSAGLPPFCHL
ALLRAEAHKPGQAESFLDEACSEAEHLLGELGLTGIELLGPVPAPMERRAGRIRAQLLLQSNARAPLHRLLNHWLHALEQ
MPSGRAVRWSLDVDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=730615 N5O83_RS02240 WP_279534274.1 473329..475548(-) (priA) [Pseudomonas oleovorans strain GD04132]
TTGCCCAACCTGATCCTGCGCCTCGCCCTGCCCTCGCCGCTGCGCCGCCTGTTCGACTACCTCGCCCCCGCCGGCGTGTC
GCGCAGCGCCTTGCAGCCAGGCGCACGCCTGCGCGTGCCCTTCGGCCGGCGCGAGATGATCGGCGTACTGGTTGAAGTCG
ACAGCCAGAGTGAGGTACCACTCGACAAGCTCAAGCCCGCCCTGGAATTGCTCGATGCTCGCCCACCGCTACCGGCGTCG
CTGTTCAAGCTGTGCCTGTGGACGGCGCAGTACTACCAGCACAGCCTCGGCGACACCCTGAGCTGGGCGTTGCCGGTACT
GCTGCGTCAGGGCGAGCCAGCCGAAACCCGCCAGGAGCGCTACTGGCTGGCCAACAAAGGCGCCAGCGTCGACGACCCCC
GCCTGGCCCGCGCGCCACGCCAGCGCGACGCGCTCAAGGCGCTGGCGCAACACCCTCATGGCGTGGCCCACAGCCTGCTC
AGCCAGCTGCAACTCAACCGCGACAGCCTGCAATTGCTGCATGAGAAGGGACTGGTACGGGTCGAGGTGCGCCGCACCCA
GCCGCATGCCAAGCCGGCGTACTGGCTGGCACAACCGGAACTGCCGCTGAACGCCGAGCAGCGCGCTGCGGTGAATGCCG
TGGCGTCAGGCTGGAGCCAGTTCAATGCCTTTCTACTGGCTGGCGTCACCGGCAGCGGCAAAACCGAGGTGTACCTGCAA
CTGATCCACCAATGCCTGGAGGCCGGCAAGCAGGCGCTGGTGCTGATCCCCGAGATCAACCTCGGCCCACAAACCTTCGA
CCGTTTCGCCCGCCGCTTCAATGCACGTATCGCCCTGCTGCATTCGGCGGTCAACGACCGCGAGCGCCTCGACGCCTGGC
TGGCGGCACGCGATGGCGAGGCCGACATCATCATCGGCACGCGCTCGGCTCTGTTCACGCCGATGAAGAACCCGGGGCTG
ATCATCGTCGACGAAGAACACGACGCCTCCTATAAACAGCAGGAGGGTCTGCGCTACCACGCCCGCGACCTGGCGCTGGT
GCGCGCGCGCCAGGAAGACGTGCCCATCGTCCTCGGCTCGGCTACACCTTCGCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTATGCCCTGCTCAAGCTGACCCAGCGCGCCGGCGGCGCCAGCCAGCCGCGCTTCCTGCGCCTGGACGTGAAGAGC
CGGCCACTGGACTCGGGCATCTCCGGGCCGATGCAGCAGGCCATCGCCCAGACCCTCGCCGCCGGCCAGCAGGTGCTGGT
ATTTCTCAACCGCCGCGGCTTCGCCCCGACCCTGCTCTGCCACGACTGCGGCTGGCTGTCCGAATGCCCACGCTGCGACG
CACGCATGACCGTGCACCAGCGCTATCAGGAACTGCGCTGCCACCACTGTGGCCATGTCGAGCGGCAGCCGAGCAACTGT
CCAAAATGCCAGCACCTCGACCTGCGTCCCGTCGGCGCCGGCACCGAGCGCGCCGAAGAGCGCCTGGCGGTGCTGTTTCC
CGACTACCCGGTGCTGCGCGTCGACCGCGACAGCACCTCGCGCAAGGGCGCCATGGATCGCCTGTTCGCCACCATCAACA
AGGGCAAGCCGTGCATCCTGGTCGGCACGCAGATGCTCGCCAAGGGGCATCACTTTCCACGGGTGACGCTGGTGTCGATC
CTCGACGCCGACGGCGGCCTGTTTTCGGCCGACTTTCGCGCCAGCGAGCGCATGGCGCAGCTGATCGTGCAGGTTGCCGG
TCGCGCTGGCCGTGCCGAGGAACCGGGCAAGGTGATCATCCAGAGCCACCTGGCCGACCATCCGCTACTGGTGCAACTGA
CCGAGCAAGGCTACTTCGCCTTCGCCGAACAGGCGCTGAGCGAACGCCGCAGTGCCGGTCTGCCACCGTTCTGCCACTTG
GCGTTGCTGCGCGCCGAAGCGCACAAGCCAGGCCAGGCGGAAAGCTTTCTCGATGAGGCCTGCAGTGAGGCCGAACATTT
GCTGGGCGAACTGGGTCTGACCGGCATCGAACTGCTCGGCCCGGTCCCTGCGCCGATGGAGCGCCGCGCCGGGCGCATCC
GCGCGCAGCTGCTGCTGCAGAGCAATGCCCGTGCGCCATTGCACCGGCTGCTCAACCACTGGCTGCATGCGCTGGAACAA
ATGCCCAGCGGCCGTGCGGTACGCTGGTCGCTGGATGTCGACCCGATCGATCTTTTCTGA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

DEAD/DEAH box helicase (DEAD)

(207-367)

Helicase conserved C-terminal domain (Helicase_C)

(525-592)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

48.37

99.594

0.482