Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N4261_RS24810 Genome accession   NZ_CP104562
Coordinates   5998204..5999751 (+) Length   515 a.a.
NCBI ID   WP_261757914.1    Uniprot ID   -
Organism   Roseateles amylovorans strain BIM B-1768     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5993204..6004751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4261_RS24795 (N4261_24820) - 5993328..5996327 (+) 3000 WP_261757910.1 polysaccharide deacetylase family protein -
  N4261_RS24800 (N4261_24825) lptA 5996804..5997424 (+) 621 WP_261757912.1 lipopolysaccharide transport periplasmic protein LptA -
  N4261_RS24805 (N4261_24830) lptB 5997421..5998188 (+) 768 WP_261757913.1 LPS export ABC transporter ATP-binding protein -
  N4261_RS24810 (N4261_24835) rpoN/rpoN1 5998204..5999751 (+) 1548 WP_261757914.1 RNA polymerase factor sigma-54 Machinery gene
  N4261_RS24815 (N4261_24840) - 5999873..6001168 (+) 1296 WP_261757915.1 THUMP domain-containing protein -
  N4261_RS24820 (N4261_24845) - 6001303..6002094 (-) 792 WP_261757916.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N4261_RS24825 (N4261_24850) - 6002231..6002797 (-) 567 WP_261757917.1 NAD(P)H-dependent oxidoreductase -
  N4261_RS24830 (N4261_24855) - 6002856..6003398 (-) 543 WP_261757918.1 hypothetical protein -

Sequence

Protein


Download         Length: 515 a.a.        Molecular weight: 56947.90 Da        Isoelectric Point: 4.5788

>NTDB_id=730601 N4261_RS24810 WP_261757914.1 5998204..5999751(+) (rpoN/rpoN1) [Roseateles amylovorans strain BIM B-1768]
MRPTLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLASNPLLETDEEHDAPLPAATQERLTEREEREERSAGTTE
GGVGESTGETSGDGAAADEIRAEDFGTTERDDWENGTEGDDFDGIREQPAGASPGSGGDDDERPETEAPDASLQDVLRAQ
MAGMQLDEVERAALLALIESLNDDGYLEDTLEELVVALLGEDGDEEQREQLLAHFKCALKWLQSMEPAGVGARELSECLE
LQLRTHPRSPEQALAIVICRRHLELLAKRDWRRLMSLTGADDELLRGAQALISGLEPKPGRRYARNDNRPVVPDVIVQKA
GRNFRVMINPDVLPKLRINDLYAQALRATRGGVSNSPLGGQLQEARWFIKNIQQRFDTILRVSEAIVERQRGFFTHGELA
MKPLVLREIADELGLHESTISRVTTAKYMATPWGTFELKYFFGSSLSTEAGGNASSTAVRALIKQFIAAEDTTSPLSDSQ
LSTMLGEQGITVARRTVAKYREALKLAPANLRKAR

Nucleotide


Download         Length: 1548 bp        

>NTDB_id=730601 N4261_RS24810 WP_261757914.1 5998204..5999751(+) (rpoN/rpoN1) [Roseateles amylovorans strain BIM B-1768]
ATGAGGCCGACGCTACAGGTACGCCTGTCCCAGCATCTGGCGCTGACGCCTCAGCTCCAGCAGTCCATCCGTCTGCTGCA
GTTGTCGACGCTGGAACTGCACCAGGAAGTGGAGCAGATGCTGGCCAGCAATCCGCTGCTCGAGACCGATGAAGAGCATG
ACGCCCCGTTGCCCGCCGCCACCCAGGAACGCCTGACCGAACGCGAAGAGCGCGAAGAGCGCAGCGCAGGCACGACCGAG
GGCGGCGTGGGCGAGAGCACCGGGGAAACTTCCGGCGACGGGGCAGCGGCCGACGAGATCCGCGCCGAGGACTTCGGCAC
CACCGAGCGCGACGACTGGGAAAACGGCACCGAGGGTGACGACTTCGACGGCATCCGCGAGCAGCCCGCAGGCGCCTCGC
CGGGAAGCGGCGGCGACGACGATGAGCGCCCGGAGACCGAGGCGCCCGACGCGTCGCTCCAGGACGTGCTGCGCGCGCAG
ATGGCCGGCATGCAGCTCGACGAGGTCGAGCGCGCCGCGCTGTTGGCGCTGATCGAGTCCCTCAACGACGACGGCTACCT
GGAAGACACGCTGGAAGAGCTGGTGGTCGCCTTGCTGGGCGAGGACGGCGACGAGGAGCAGCGCGAGCAGTTGCTCGCCC
ACTTCAAATGTGCGCTCAAGTGGCTGCAATCGATGGAGCCCGCCGGTGTCGGGGCGCGCGAGCTGTCGGAATGCCTGGAG
CTGCAGCTTCGCACCCATCCGCGATCGCCCGAGCAGGCGCTGGCGATCGTCATCTGCCGACGCCACCTCGAGTTGCTGGC
CAAGCGCGACTGGCGCCGCCTGATGTCCCTGACCGGCGCGGACGACGAGTTGCTGCGCGGTGCGCAGGCCTTGATCTCCG
GTCTGGAGCCCAAGCCCGGCCGGCGCTATGCCCGCAATGACAACCGGCCGGTGGTGCCCGATGTGATCGTGCAGAAGGCC
GGTCGAAACTTCCGGGTGATGATCAATCCGGATGTGCTGCCCAAGCTGCGCATCAATGATCTCTATGCCCAGGCCCTGCG
GGCGACCCGGGGCGGCGTGAGCAACTCGCCCCTGGGTGGCCAGTTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGC
AGCGCTTCGACACCATCCTGCGGGTCAGCGAGGCGATCGTCGAACGCCAGCGCGGTTTCTTCACCCATGGCGAACTGGCG
ATGAAACCGCTGGTGCTCCGCGAGATCGCCGATGAGCTGGGTCTGCATGAATCGACGATCTCCCGGGTCACGACCGCCAA
GTACATGGCCACGCCCTGGGGCACCTTCGAGCTGAAATACTTCTTCGGCTCCAGTCTCTCCACCGAAGCCGGCGGCAATG
CCTCCAGCACCGCCGTGCGGGCGTTGATCAAGCAGTTCATTGCGGCGGAGGACACCACCAGTCCGCTGTCGGACAGCCAG
TTGTCGACCATGCTTGGCGAGCAGGGCATCACCGTGGCCCGGCGCACCGTCGCCAAGTACCGCGAGGCGCTCAAGCTCGC
GCCCGCGAACCTGCGCAAGGCCCGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(148-342)

Sigma-54, DNA binding domain (Sigma54_DBD)

(361-513)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.838

100

0.52